DSpace About DSpace Software 日本語
 

GINMU >
01 奈良県立医科大学 >
012 大学院 >
0122 学位請求論文 >
01221 博士論文(医学) >
2022年度 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/4104

Title: Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
Other Titles: 脳皮質静脈梗塞ラットモデルを用いた脳静脈虚血による神経細胞および神経前駆細胞の発現と分布
Authors: Kim, Tae-Kyun
Takeshima, Yasuhiro
Ouji, Yukiteru
Nishimura, Fumihiko
Nakagawa, Ichiro
Young-Soo, Park
Yoshikawa, Masahide
Nakase, Hiroyuki
Keywords: Cerebral ischemia
Cerebral venous infarction
Endogenous precursors
Neural stem cells
Neurogenesis
Issue Date: 13-Dec-2022
Publisher: Elsevier
Citation: IBRO neuroscience reports Vol.14 p.50-56 (2022 Dec)
Abstract: Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI rat models via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by doublelabeling 5-bromo-2′ -deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX) antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separated into two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefined region of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we divided the ROI into six sections to evaluate and compare the distribution of double-positive cells generated in each section between the two subgroups. The 2-VO subgroup presented more double-positive cells than the sham group in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristic distribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra, with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has the potential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons and precursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be the main origin of neurogenesis after cortical CVI.
Description: 博士(医学)・甲第872号・令和5年3月15日
© 2022 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/10564/4104
ISSN: 26672421
DOI: https://doi.org/10.1016/j.ibneur.2022.12.005
Academic Degrees and number: 24601甲第872号
Degree-granting date: 2023-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2022年度

Files in This Item:

File Description SizeFormat
01甲872本文の要旨.pdf72.31 kBAdobe PDFView/Open
02甲872審査要旨.pdf290.9 kBAdobe PDFView/Open
03甲872本文.pdf3.53 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback