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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3736

Title: Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice.
Other Titles: アルサニル酸を用いたマウス内耳破壊後の前庭代償過程
Authors: Ito, Taeko
Tatsumi, Kouko
Takimoto, Yasumitsu
Nishimura, Tadashi
Imai, Takao
Yamanaka, Toshiaki
Takeda, Noriaki
Wanaka, Akio
Kitahara, Tadashi
Keywords: mouse
labyrinthectomy
p-arsanilic acid
c-Fos
Arc
Zif268
vestibular nucleus
head deviation
nystagmus
vestibular compensation
Issue Date: 18-Nov-2019
Publisher: MDPI
Citation: Brain sciences Vol.9 No.11 Article No.329 (2019 Nov)
Abstract: When vestibular function is lost, vestibular compensation works for the reacquisition of body balance. For the study of vestibular dysfunction and vestibular compensation, surgical or chemical labyrinthectomy has been performed in various animal species. In the present study, we performed chemical labyrinthectomy using arsanilic acid in mice and investigated the time course of vestibular compensation through behavioral observations and histological studies. The surgical procedures required only paracentesis and storage of 50 µL of p-arsanilic acid sodium salt solution in the tympanic cavity for 5 min. From behavioral observations, vestibular functions were worst at 2 days and recovered by 7 days after surgery. Spontaneous nystagmus appeared at 1 day after surgery with arsanilic acid and disappeared by 2 days. Histological studies revealed specific damage to the vestibular endorgans. In the ipsilateral spinal vestibular nucleus, the medial vestibular nucleus, and the contralateral prepositus hypoglossal nucleus, a substantial number of c-Fos-immunoreactive cells appeared by 1 day after surgery with arsanilic acid, with a maximum increase in number by 2 days and complete disappearance by 7 days. Taken together, these findings indicate that chemical labyrinthectomy with arsanilic acid and the subsequent observation of vestibular compensation is a useful strategy for elucidation of the molecular mechanisms underlying vestibular pathophysiologies.
Description: 博士(医学)・甲第742号・令和2年3月16日
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/10564/3736
ISSN: 20763425
Academic Degrees and number: 24601A742
Degree-granting date: 2020-03-16
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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