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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3717

Title: Novel Human NK Cell Line Carrying CAR Targeting EGFRvIII Induces Antitumor Effects in Glioblastoma Cells.
Other Titles: EGFRvIII特異的キメラ抗原受容体発現NK細胞を用いたGBMに対する新規治療法の開発
Authors: Murakami, Toshiharu
Nakazawa, Tsutomu
Natsume, Atsushi
Nishimura, Fumihiko
Nakamura, Mitsutoshi
Matsuda, Ryosuke
Omoto, Koji
Tanaka, Yoshitaka
Shida, Youichi
Park, Young-Soo
Motoyama, Yasushi
Nakagawa, Ichiro
Yamada, Shuichi
Tamura, Kentaro
Takeshima, Yasuhiro
Takamura, Yoshiaki
Wakabayashi, Toshihiko
Nakase, Hiroyuki
Keywords: Chimeric antigen receptor
natural killer cells
epidermal growth factor receptor variant III
Issue Date: Sep-2018
Publisher: International Institute of Anticancer Research
Citation: Anticancer research Vol.38 No.9 p.5049-5056 (2018 Sep)
Abstract: Background/Aim: Natural killer (NK) cells are considered potential antitumor effector cells. The aim of this study was toestablish a novel type of a chimeric antigen receptor (CAR) NK cell line (CAR-KHYG-1) specific for epidermal growth factorreceptor variant III (EGFRvIII)-expressing tumors and investigate the anti-tumor activity of EGFRvIII-specific-CAR-KHYG-1(EvCAR-KHYG-1). Materials and Methods: EvCAR-KHYG-1 was established by self-inactivated lentiviral-based transduction of theEvCAR gene and magnetic bead-based purification of EvCAR-expressing NK cells. The anti-tumor effects of EvCAR-KHYG-1 wereevaluated using growth inhibition and apoptosis detection assays in glioblastoma (GBM) cell lines (EGFRvIII-expressing andnon-expressing U87MG). Results: The findings demonstrated that EvCAR-KHYG-1 inhibited GBM cell-growth via apoptosis in anEGFRvIII-expressing specific manner. Conclusion: This is the first study to establish a CAR NK cell line based on the humanNK cell line KHYG-1. Therapy with EvCAR-KHYG-1 may be an effective treatment option for GBM patients.
Description: 博士(医学)・甲第726号・令和2年3月16日
Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
発行元の規定により、本文の登録不可。本文は以下のURLを参照 "http://dx.doi.org/10.21873/anticanres.12824"(※全文閲覧は学内限定)
URI: http://hdl.handle.net/10564/3717
ISSN: 02507005
Academic Degrees and number: 24601A726
Degree-granting date: 2020-03-16
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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