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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3587

Title: Expression of peptide transporter 1 has a positive correlation in protoporphyrin IX accumulation induced by 5-aminolevulinic acid with photodynamic detection of non-small cell lung cancer and metastatic brain tumor specimens originating from non-small cell lung cancer.
Other Titles: 非小細胞肺がんと非小細胞肺がんから発生した転移性脳腫瘍における光線力学的測定によりPEPT1の発現は5-ALAから代謝されるプロトポルフィリンⅨの蓄積において正の相関をする
Authors: Omoto, Koji
Matsuda, Ryosuke
Nakai, Yasushi
Tatsumi, Yoshihiro
Nakazawa, Tsutomu
Tanaka, Yoshitaka
Shida, Yoichi
Murakami, Toshiharu
Nishimura, Fumihiko
Nakagawa, Ichiro
Motoyama, Yasushi
Nakamura, Mitsutoshi
Fujimoto, Kiyohide
Nakase, Hiroyuki
Keywords: Protoporphyrin Ⅸ
Peptide transporter 1
Non-small lung cancer
Brain
Metastasis
Issue Date: Mar-2019
Publisher: Elsevier
Citation: Photodiagnosis and photodynamic therapy Vol.25 p.309-316 (2019 Mar)
Abstract: BACKGROUND: Recently, 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX fluorescence was reported to be a useful tool during total surgical resection of high-grade gliomas. However, the labeling efficacy of protoporphyrin IX fluorescence is lower in metastatic brain tumors compared to that in high-grade gliomas, and the mechanism underlying protoporphyrin IX fluorescence in metastatic brain tumors remains unclear. Lung cancer, particularly non-small cell lung cancer (NSCLC), is the most common origin for metastatic brain tumor. Therefore, we investigated the mechanism of protoporphyrin IX fluorescence in NSCLC and associated metastatic brain tumors. METHODS: Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) was employed to evaluate the protein and mRNA levels of five transporters and enzymes involved in the porphyrin biosynthesis pathway: peptide transporter 1 (PEPT1), hydroxymethylbilane synthase (HMBS), ferrochelatase (FECH), ATP-binding cassette 2 (ABCG2), and heme oxygenase 1 (HO-1). The correlation between protein, mRNA, and protoporphyrin IX levels in NSCLC cells were evaluated in vitro. Immunohistochemistry was used to determine proteins that played a key role in intraoperative protoporphyrin IX fluorescence in clinical samples from patients with NSCLC and pathologically confirmed metastatic brain tumors. RESULTS: A significant correlation between PEPT1 expression and protoporphyrin IX accumulation in vitro was identified by western blotting (P = 0.003) and qRT-PCR (P = 0.04). Immunohistochemistry results indicated that there was a significant difference in PEPT1 between the intraoperative protoporphyrin IX fluorescence-positive and protoporphyrin IX fluorescence-negative groups (P = 0.009). CONCLUSION: Expression of PEPT1 was found to be positively correlated with 5-ALA-induced protoporphyrin IX accumulation detected by photodynamic reaction in metastatic brain tumors originating from NSCLC.
Description: 博士(医学)・甲第714号・令和元年年6月26日
Copyright © 2019 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/10564/3587
ISSN: 15721000
Academic Degrees and number: 24601A714
Degree-granting date: 2019-06-26
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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