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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3321

Title: Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats.
Other Titles: 骨形成シートによる顎顔面の再生 : ラットにおける実験研究
Authors: Ueyama, Yoshihiro
Yagyuu, Takahiro
Maeda, Masahiko
Imada, Mitsuhiko
Akahane, Manabu
Kawate, Kenji
Tanaka, Yasuhito
Kirita, Tadaaki
Keywords: Bone regeneration
Mandibular defect
Bone marrow-derived stromal cells
Cell sheet
Issue Date: Dec-2016
Publisher: Elsevier
Citation: Archives of oral biology Vol.72 p.138-145 (2016 Dec)
Abstract: OBJECTIVE: Regeneration of maxillofacial bone defects, characterized by relatively small but complicated shapes, poses a significant clinical challenge. Osteogenic matrix cell sheets (OMCSs) have osteogenic ability and good shaping properties and may be ideal graft materials. Here, we assessed whether implantation of OMCSs could be used to repair maxillofacial bone defects. DESIGN: We adopted a rat mandibular symphysis model. The rat mandible is formed by a paired bone and the central portion consisting of fibrous tissue. There is no bone tissue at the site; accordingly, this site was interpreted as a physiological bone gap and was used for evaluation. Rat bone marrow cells were cultured in medium containing dexamethasone and ascorbic acid phosphate to create OMCSs. The OMCSs were implanted into the rat mandibular symphysis without a scaffold. Microcomputed tomography and histological analyses were conducted after 2, 4, and 8 weeks. RESULTS: Two weeks after implantation, microcomputed tomography images and histological sections showed some sparse granular calcification tissue within the bone gap at the mandibular symphysis. At 4 weeks, the calcification tissue spread, and the gap of the mandibles were continued. At 8 weeks, this continuous new bone tissue was matured. The experimental group showed abundant new bone tissue in the group with OMCS implantation, but not in the group with sham implantation. CONCLUSIONS: Our present results indicated that use of OMCSs may be an optimal approach towards achieving maxillofacial regeneration.
Description: 博士(医学)・甲第668号・平成29年3月15日
Copyright © 2016 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/10564/3321
ISSN: 00039969
Academic Degrees and number: 24601A668
Degree-granting date: 2017-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2016年度

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