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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3190

Title: Promotion of osteogenesis and angiogenesis in vascularized tissue-engineered bone using osteogenic matrix cell sheets.
Other Titles: 骨形成細胞シートは血管柄付き人工骨内での骨形成および血管形成を促進させる
Authors: Nakano, Kenichi
Murata, Keiichi
Omokawa, Shohei
Akahane, Manabu
Shimizu, Takamasa
Kawamura, Kenji
Kawate, Kenji
Tanaka, Yasuhito
Issue Date: 2016
Publisher: Lippincott Williams & Wilkins
Citation: Plastic and reconstructive surgery Vol.137 No.5 p.1476-1484(2016 May)
Abstract: BACKGROUND: The regeneration of large, poorly vascularized bone defects remains a significant challenge. Although vascularized bone grafts promote osteogenesis, the required tissue harvesting causes problematic donor-site morbidity. Artificial bone substitutes are promising alternatives for regenerative medicine applications, but the incorporation of suitable cells and/or growth factors is necessary for their successful clinical application. The inclusion of vascular bundles can further enhance the bone-forming capability of bone substitutes by promoting tissue neovascularization. Little is known about how neovascularization occurs and how new bone extends within vascularized tissue-engineered bone, because no previous studies have used tissue-engineered bone to treat large, poorly vascularized defects. METHODS: In this study, the authors developed a novel vascularized tissue-engineered bone scaffold composed of osteogenic matrix cell sheets wrapped around vascular bundles within β-tricalcium phosphate ceramics. RESULTS: Four weeks after subcutaneous transplantation in rats, making use of the femoral vascular bundle, vascularized tissue-engineered bone demonstrated more angiogenesis and higher osteogenic potential than the controls. After vascularized tissue-engineered bone implantation, abundant vascularization and new bone formation were observed radially from the vascular bundle, with increased mRNA expression of alkaline phosphatase, bone morphogenetic protein-2, osteocalcin, and vascular endothelial growth factor-A. CONCLUSION: This novel method for preparing vascularized tissue-engineered bone scaffolds may promote the regeneration of large bone defects, particularly where vascularization has been compromised.
Description: 博士(医学)・甲第652号・平成28年3月15日
Copyright © 2016 American Society of Plastic Surgeons All rights reserved.
This is a non-final version of an article published in final form in "http://dx.doi.org/10.1097/PRS.0000000000002079"
URI: http://hdl.handle.net/10564/3190
ISSN: 00321052
Academic Degrees and number: 24601A652
Degree-granting date: 2016-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2015年度

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