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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3187

Title: Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction.
Other Titles: 凍結保存骨形成細胞シートの骨再建における有用性
Authors: Kura, Tomohiko
Akahane, Manabu
Shimizu, Takamasa
Uchihara, Yoshinobu
Tohma, Yasuaki
Morita, Yusuke
Koizumi, Munehisa
Kawate, Kenji
Tanaka, Yasuhito
Keywords: Bone Marrow Stromal Cell
Cell Sheet
Injectable Bone
Bone Reconstruction
Issue Date: Jan-2016
Publisher: Scientific Research Publishing Inc.
Citation: Stem Cell Discovery Vol.6 No.1 p.13-23(2016.01)
Abstract: Skeletal diseases, such as nonunion and osteonecrosis, are now treatable with tissue engineering techniques. Single cell sheets called osteogenic matrix cell sheets (OMCSs) grown from cultured bone marrow-derived mesenchymal stem cells show high osteogenic potential; however, long preparation times currently limit their clinical application. Here, we report a cryopreservation OMCS transplantation method that shortens OMCS preparation time. Cryopreserved rat OMCSs were prepared using slow- and rapid-freezing methods, thawed, and subsequently injected scaffold-free into subcutaneous sites. Rapid- and slow-frozen OMCSs were also transplanted directly to the femur bone at sites of injury. Slow-freezing resulted in higher cell viability than rapid freezing, yet all two cryopreservation methods yielded OMCSs that survived and formed bone tissue. In the rapid- and slow-freezing groups, cortical gaps were repaired and bone continuity was observed within 6 weeks of OMCS transplantation. Moreover, while no significant difference was found in osteocalcin expression between the three experimental groups, the biomechanical strength of femurs treated with slow-frozen OMCSs was significantly greater than those of non-transplant at 6 weeks post-injury. Collectively, these data suggest that slow-frozen OMCSs have superior osteogenic potential and are better suited to produce a mineralized matrix and repair sites of bone injury.
Description: 博士(医学)・甲第650号・平成28年3月15日
Copyright © 2016 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/
URI: http://hdl.handle.net/10564/3187
ISSN: 21616760
Academic Degrees and number: 24601A650
Degree-granting date: 2016-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2015年度

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