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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2673

Title: Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.
Other Titles: 骨形成細胞シートを用いた家兎移植腱骨孔間の早期治癒促進
Authors: Inagaki, Yusuke
Uematsu, Kota
Akahane, Manabu
Morita, Yusuke
Ogawa, Munehiro
Ueha, Tomoyuki
Shimizu, Takamasa
Kura, Tomohiko
Kawate, Kenji
Tanaka, Yasuhito
Issue Date: 2013
Publisher: Hindawi Pub. Co.
Citation: BioMed research international Vol.2013 ID:842192
Abstract: The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (0.74 ± 0.23 N/mm(2)) than without OMCS (0.58 ± 0.15 N/mm(2)). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.
Description: 博士(医学)・甲第608号・平成25年11月27日
URI: http://hdl.handle.net/10564/2673
ISSN: 23146141
Academic Degrees and number: 24601A608
Degree-granting date: 2013-11-27
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2013年度

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