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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2672

Title: Osteogenesis of cryopreserved osteogenic matrix cell sheets
Other Titles: 冷凍保存骨芽細胞シートの骨形成能評価
Authors: Shimizu, Takamasa
Akahane, Manabu
Ueha, Tomoyuki
Kido, Akira
Omokawa, Shohei
Kobata, Yasunori
Murata, Keiichi
Kawate, Kenji
Tanaka, Yasuhito
Keywords: Bone marrow mesenchymal stem cells
Cryopreservation
Cell sheet
Hydroxyapatite
Osteogenesis
Tissue engineered bone
Issue Date: Jun-2013
Publisher: Rockville / Elsevier
Citation: Cryobiology Vol.66 No.3 p.326-332
Abstract: Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application. We previously reported a novel cell transplantation method, in which bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured to confluence and differentiated down the osteogenic lineage to form osteogenic matrix cell sheets (OMCS). OMCS have high alkaline phosphatase (ALP) activity and osteocalcin (OC) contents and can be easily used for producing TEB. The aim of the present study was to investigate whether TEB produced by cryopreserved OMCS maintains sufficient osteogenic potential in vivo. OMCS were prepared and divided into three groups according to storage period of cryopreservation (fresh (no cryopreservation), 4 week and 12 week cryopreservation groups). OMCS were cryopreserved by storage in freezing medium (Cell Banker 1®) at -80 °C. Cryopreserved OMCSs were rapidly thawed at room temperature and wrapped around Hydroxyapatite (HA) scaffolds prior to implantation into subcutaneous sites in rats, to determine their in vivo bone-forming capability. The constructs were harvested 4 weeks after transplantation and examined histologically and biochemically. Histological analysis of the constructs showed extensive bone formation in the HA pores with high ALP activity and OC content detected in the cryopreservation groups. The present study clearly indicates that cryopreserved/thawed OMCS are still capable of producing mineralized matrix on scaffolds, resulting in bone formation. This cryopreservation technique could be applied for hard tissue reconstruction to ease the cell preparation method prior to time of use.
Description: 博士(医学)・甲第607号・平成25年11月27日
URI: http://hdl.handle.net/10564/2672
ISSN: 00112240
Academic Degrees and number: 24601A607
Degree-granting date: 2013-11-27
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2013年度

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