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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2589

Title: In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr:Forsterite laser
Other Titles: 1250nm帯モード同期クロム・フォルステライトレーザーを光源とした第2高調波発生光顕微鏡によるヒト顔皮膚の老化性真皮コラーゲン構造変化のその場観察
Authors: Yasui, Takeshi
Yonetsu, Makoto
Tanaka, Ryosuke
Tanaka, Yuji
Fukushima, Shu-ichiro
Yamashita, Toyonobu
Ogura, Yuki
Hirao, Tetsuji
Murota, Hiroyuki
Araki, Tsutomu
Keywords: nonlinear microscopy
second harmonic generation
collagen
dermatology
intrinsic aging
photoaging
tissue
dermis
Issue Date: Mar-2013
Publisher: SPIE--the International Society for Optical Engineering
Citation: Journal of biomedical optics Vol.18 No.3 p.31108(1-10)
Abstract: In vivo visualization of human skin aging is demonstrated using a Cr:Forsterite (Cr:F) laser-based, collagen-sensitive second harmonic generation (SHG) microscope. The deep penetration into human skin, as well as the specific sensitivity to collagen molecules, achieved by this microscope enables us to clearly visualize age-related structural changes of collagen fiber in the reticular dermis. Here we investigated intrinsic aging and/or photoaging in the male facial skin. Young subjects show dense distributions of thin collagen fibers, whereas elderly subjects show coarse distributions of thick collagen fibers. Furthermore, a comparison of SHG images between young and elderly subjects with and without a recent life history of excessive sun exposure show that a combination of photoaging with intrinsic aging significantly accelerates skin aging. We also perform image analysis based on two-dimensional Fourier transformation of the SHG images and extracted an aging parameter for human skin. The in vivo collagen-sensitive SHG microscope will be a powerful tool in fields such as cosmeceutical sciences and anti-aging dermatology.
Description: 博士(医学)・乙第1311号・平成25年5月29日
URI: http://hdl.handle.net/10564/2589
ISSN: 10833668
Academic Degrees and number: 24601B1311
Degree-granting date: 2013-05-29
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2013年度

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