Silver methenamine and Masson's trichrome重染色による腎糸球体の観察(基底膜変化を中心に)

Abstract

In order to observe the precise morphological changes of glomeruli on light microscopic level, silver methenamine and Masson's trichrome (SM-MT) staining was employed on renal biopsy sections from children with various glomerular diseases. With the SM-MT staining, basement membrane stains black, red blood cells red, fresh fibrinoid material bright red and protein deposits are stained red in various shades depending on the composition and age of the deposits. There was a good contrast between basement membrane and other materials. These light microscopic changes reflected well the ultrastructural changes which were investigated simultaneously in several cases. Therefore, it is suggested that this staining provides valuable information about glomerular changes on routine light microscopy.