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Vol.43 No.6 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/1854

Title: 糖尿病性腎症における尿中レクチン結合性低分子糖蛋白分画
Other Titles: ELECTROPHORETIC ANALYSIS OF URINARY GLYCOPROTEIN FOR DIABETIC NEPHROPATHY USING PEROXIDASE-LECTINS
Authors: 森本, 真弓
金内, 雅夫
中島, 靖夫
西浦, 公章
土肥, 和紘
石川, 兵衞
土肥, 祥子
森山, 忠重
Keywords: electrophoretic analysis
diabetic nephropathy
lectin
urinary glycoprotein
Issue Date: 31-Dec-1992
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.43 No.6 p.598-603
Abstract: To evaluate the severity of diabetic renal involvement, we performed electrophoretic analysis of urinary glycoprotein for 16 patients with diabetic nephropathy using peroxidase-lectins. After being subjected to SDS-polyacrylamide gel electrophoresis, urinary glycoprotein was transferred to the nitrocellulose membrane, which then was stained with peroxidase labeled-lectin (Triticum vulgaris ; WGA), and was analyzed densitometrically. Electrophoretic analysis of urinary glycoprotein revealed the presence of lectin-reactive glycoproteins with the molecular weights of 22 kD, 32 kD, 38 kD and 48 kD respectively. Among these, the amount of the 22 kD-mol. wt. glycoprotein increased significantly as the renal histopathological changes developed, whereas that of 32 kD decreased. These results suggest that semiquantitative analysis of low molecular weight urinary glycoprotein stained with lectin is useful for the non-invasive evaluation of the severity of diabetic nephropathy.
URI: http://hdl.handle.net/10564/1854
ISSN: 04695550
13450069
Appears in Collections:Vol.43 No.6

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