オオバヤシャブシ花粉のアレルゲン分析

Abstract

Alder (Alnus sieboldiana) pollen contained at least 14 antigenic compo- nents, demonstrable by SDS-PAGE. One of these antigens was responsible for a major allergenic activity of alder pollen extracts. The major allergen of alder pollen was separated from other allergenic components as well as non-allergenic antigens by ion- exchange chromatography and disk preparative electrophoresis. Forty-two sera from patients with alder pollen allergy were investigated for the presence of IgE antibodies against alder pollen allergens by means of immunoblotting. In immuno- blots, 38 patients (90%) exhibited IgE antibodies against the major allergen of alder pollen with a molecular mass of 17 kDa. The pⅠ value of the major allergen determined by preparative isoelectric focusing was 5.7. The major allergen was stable to heat, acidic and alkaline treatments. The N-terminal amino acid sequence of the major allergen was Gly-Val-Phe-Asn-Tyr- Glu-His-Glu-Thr-Pro-. The N-terminal amino acid sequences of the major allergens of A. sieboldiana pollen, A. glutinosa (Aln g 1) and Betula verrucosa (Bet v 1) exhibited a high degree of homology.