口腔扁平上皮癌におけるMatrix metalloproteinaseとTissue inhibitor of metalloproteinaseの発現と局在についての検討

Abstract

Immunohistochemical stainings of matrix metalloproteinase (MMP)-2, 9 and tissue inhibitor of metalloproteinase (TIMP)-1, 2, 3 with specific antibodies in paraffin sections of 14 human oral squamous cell carcinomas and 8 normal tissues were perfomed by labeled streptavidinbiotin method. The expression and localization of mRNA of TIMP-1, 2, 3 were studied by in situ hybridization with digoxigenin (DIG) RNA probe in the same specimens. Positive immunostaining for MMP-2, 9 and TIMP-1 were detected in stromal cells of carcinoma, whereas positive staining for TIMP-3 was detected in both stromal cells and cytoplasms of carcinoma cells. Diffuse expression of MMP-2, 9 was observed in carcinomas with advanced stages. They were not detected in normal tissues. Expressions of TIMP-3 mRNA were observed in 11 out of 14 carcinomas. They were seen in stromal cells and in cytoplasms of cardinoma cells (7/4 in only stromal cells and 4/14 in both stromal cells and carcinoma cells). Carcinomas expressing TIMP-3 mRNA tended to be smaller in size than those which were non-expressing. TIMP-3 mRNA was not detected in normal tissues. Our results suggest that MMP-2, 9 are important for the development of oral squamous cell carcinoma and TIMP-3 is an important inhibitor of it.