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01121 Journal of Nara Medical Association >
Vol.49 No.6 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/480
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タイトル: | オオバヤシャブシ花粉主アレルゲンのクローニングと大腸菌での発現 |
その他のタイトル: | CLONING, SEQUENCING AND EXPRESSION IN Escherichia coli OF THE MAJOR ALLERGEN FROM ALDER (Alnus sieboldiana) POLLEN |
著者: | 吉川, 恒男 |
キーワード: | alder pollen cDNA cloning expression major allergen recombinant allergen |
発行日: | 1998年12月31日 |
出版者: | 奈良医学会 |
引用: | Journal of Nara Medical Association Vol.49 No.6 p.453-464 |
抄録: | Pollen of the alder (Alnus sieboldiana) is one of the main causes of Type
Ⅰ allergies in Japan, especially in densely cultivated areas. cDNA clones coding for the
major alder pollen allergen, Aln s 1, were isolated. The clones contained an open reading
frame of 477 nucleotides. Comparison of the deduced amino acid sequence of Aln s 1 to the
major allergens from Betulaceae pollen such as Aln g 1, Bet v 1, Cor a 1 and Car b 1 showed
identities of 95.8%, 87.6%, 85.1%, and 85.7%, respectively. The Alns 1-encoding cDNA
was ligated into expression plasmid, pKP 1500, and expressed in Escherichia coli as a full-
length nonfusion protein. The recombinant protein was able to bind specific IgE antibodies
present in sera of alder pollen-allergic subjects. However, COOH terminally truncated
form of Aln s 1 had no IgE-binding capacity. |
URI: | http://hdl.handle.net/10564/480 |
ISSN: | 13450069 |
出現コレクション: | Vol.49 No.6
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