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タイトル: MgO‑enhanced β‑TCP promotes osteogenesis in both in vitro and in vivo rat models
その他のタイトル: MgO添加β-TCPはin vitroおよびin vivoのラットモデルにおいて骨形成を促進する
著者: Saito, Kenichiro
Inagaki, Yusuke
Uchihara, Yoshinobu
Okamoto, Masakazu
Nishimura, Yuki
Kawai, Akihito
Sugino, Tatsuro
Okamura, Kensuke
Ogawa, Munehiro
Kido, Akira
Tanaka, Yasuhito
キーワード: Allogeneic bone grafts
β-tricalcium phosphate
Bone marrow-derived mesenchymal stem cells osteogenesis
発行日: 2024年8月25日日曜日
出版者: Springer Nature
引用: Scientific reports. 2024 Aug, vol.14, no.1, article no.19725
抄録: Allogeneic bone grafts are used to treat bone defects in orthopedic surgery, but the osteogenic potential of artificial bones remains a challenge. In this study, we developed a β-tricalcium phosphate (β-TCP) formulation containing MgO, ZnO, SrO, and SiO2 and compared its bone-forming ability with that of β-TCP without biological elements. We prepared β-TCP discs with 60% porosity containing 1.0 wt% of these biological elements. β-TCP scaffolds were loaded with bone marrow-derived mesenchymal stem cells (BMSC) from 7-week-old male rats and cultured for 2 weeks. ALP activity and mRNA expression of osteogenic markers were evaluated. In addition, scaffolds were implanted subcutaneously in rats and analyzed after 7 weeks. In vitro, the MgO group showed lower Ca concentrations and higher osteogenic marker expression compared to controls. In vivo, the MgO group showed higher ALP activity compared to controls, and RT-qPCR analysis showed significant expression of BMP2 and VEGF. Histopathology, fluorescent immunostaining, and micro-CT also showed relatively better bone formation in the MgO group. β-TCP with MgO may enhance bone morphology in vitro and in vivo and improve the prognosis of patients with substantial and refractory bone defects.
内容記述: 権利情報:© The Author(s) 2024. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creativecommons.org/licenses/by-nc-nd/4.0/.
URI: http://hdl.handle.net/10564/4454
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-024-70512-5
学位授与番号: 24601甲第959号
学位授与年月日: 2025-03-14
学位名: 博士(医学)
学位授与機関: 奈良県立医科大学
出現コレクション:2024年度

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