DSpace DSpace Softwareについて English
 

GINMU >
01 奈良県立医科大学 >
012 大学院 >
0122 学位請求論文 >
01221 博士論文(医学) >
2024年度 >

このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10564/4441

タイトル: MEK inhibitor PD0325901 upregulates CD34 expression in endothelial cells via inhibition of ERK phosphorylation
その他のタイトル: MEK阻害剤PD0325901はERKリン酸化阻害を介して 内皮細胞におけるCD34発現を上昇させる
著者: Hosoda, Chihiro
Mitani, Seiji
Sakata, Asuka
Kasuda, Shogo
Onodera, Yu
Takabayashi, Yoko
Shima, Midori
Tatsumi, Kohei
キーワード: CD34
Endothelial cell
Endothelial progenitor cell
PD0325901
Reprogramming
発行日: 2024年8月29日木曜日
出版者: Elsevier
引用: Regenerative therapy. 2024 Aug, vol.26, p.654-662
抄録: CD34-positive endothelial progenitor cells (EPCs) promote angiogenesis and are a promising tool for regenerative cell therapy of ischemic diseases. However, the number and quality of CD34-positive cells decrease owing to various external and internal factors; thus, an efficient method is needed to establish CD34-positive EPCs. The generation of functional cells by reprogramming, that is, manipulating cell fate via gene transfer and/or treatment with chemical compounds, has recently been reported. Therefore, we aimed to generate CD34-positive cells by the reprogramming of endothelial cells (ECs). Methods: Based on previous reports, seven candidate chemical compounds were selected to reprogram human umbilical vein ECs (HUVECs) to CD34-positive cells. Following stimulation with the chemical compounds, the expression of CD34 was evaluated using quantitative PCR, flow cytometry, and immunocytochemistry. Results: HUVECs treated with the compounds exhibited increased CD34 expression. We cultured cells in alternate media lacking one of the seven compounds and found no CD34 expression in cells treated with PD0325901-free media, suggesting that PD0325901da MEK inhibitordmainly contributed to the increase in CD34 expression. We found that 98% of cells were CD34-positive after PD0325901 treatment alone for 7 d. Western blotting revealed that the phosphorylation of ERK was suppressed in PD0325901- treated cells. No upregulation of CD34 was observed in fibroblast cell lines, even after PD0325901 treatment. These results suggested that PD0325901 induces CD34-positive cells by inhibiting ERK phosphorylation in ECs. Conclusions: CD34 expression was strongly induced in ECs by treatment with the MEK inhibitor PD0325901 in vitro. Our study provides a useful reference for the establishment of CD34-positive EPCs and will contribute to the development of regenerative therapies, especially for ischemic diseases.
内容記述: © 2024 The Author(s). Published by Elsevier BV on behalf of The Japanese Society for Regenerative Medicine. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/10564/4441
ISSN: 2352-3204
DOI: https://doi.org/10.1016/j.reth.2024.08.009
学位授与番号: 24601甲第946号
学位授与年月日: 2025-03-14
学位名: 博士(医学)
学位授与機関: 奈良県立医科大学
出現コレクション:2024年度

このアイテムのファイル:

ファイル 記述 サイズフォーマット
02甲946審査要旨.pdf甲946審査要旨134.55 kBAdobe PDF見る/開く
03甲946本文の要旨.pdf甲946本文の要旨122.99 kBAdobe PDF見る/開く

このリポジトリに保管されているアイテムは、他に指定されている場合を除き、著作権により保護されています。

 

Valid XHTML 1.0! Powered by DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - ご意見をお寄せください