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このアイテムの引用には次の識別子を使用してください: http://hdl.handle.net/10564/4351

タイトル: Characterization of Glial Populations in the Aging and Remyelinating Mouse Corpus Callosum
その他のタイトル: マウス脳梁グリア細胞の加齢や再ミエリン化に対する反応解析
著者: Kawabe, Yoshie
Tanaka, Tatsuhide
Isonishi, Ayami
Nakahara, Kazuki
Tatsumi, Kouko
Wanaka, Akio
キーワード: Corpus callosum
Oligodendrocyte
Microglia
Astrocyte
Remyelination
発行日: 2022年9月
出版者: Springer Nature
引用: Neurochemical Research. 2022 Sep, vol.47, no.9, p.2826-2838
抄録: Cells in the white matter of the adult brain have a characteristic distribution pattern in which several cells are contiguously connected to each other, making a linear array (LA) resembling pearls-on-a-string parallel to the axon axis. We have been interested in how this pattern of cell distribution changes during aging and remyelination after demyelination. In the present study, with a multiplex staining method, semi-quantitative analysis of the localization of oligodendrocyte lineage cells (oligodendrocyte progenitors, premyelinating oligodendrocytes, and mature oligodendrocytes), astrocytes, and microglia in 8-week-old (young adult) and 32-week-old (aged) corpus callosum showed that young adult cells still include immature oligodendrocytes and that LAs contain a higher proportion of microglia than isolated cells. In aged mice, premyelinating oligodendrocytes were decreased, but microglia continued to be present in the LAs. These results suggest that the presence of microglia is important for the characteristic cell localization pattern of LAs. In a cuprizone-induced demyelination model, we observed re-formation of LAs after completion of cuprizone treatment, concurrent with remyelination. These re-formed LAs again contained more microglia than the isolated cells. This finding supports the hypothesis that microglia contribute to the formation and maintenance of LAs. In addition, regardless of the distribution of cells (LAs or isolated cells), astrocytes were found to be more abundant than in the normal corpus callosum at 24 weeks after cuprizone treatment when remyelination is completed. This suggests that astrocytes are involved in maintaining the functions of remyelinated white matter.
内容記述: 権利情報:© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022. This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s11064-022-03676-z
URI: http://hdl.handle.net/10564/4351
ISSN: 0364-3190
DOI: https://doi.org/10.1007/s11064-022-03676-z
学位授与番号: 24601甲第900号
学位授与年月日: 2024-03-14
学位名: 博士(医学)
学位授与機関: 奈良県立医科大学
出現コレクション:2023年度

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01甲900本文.pdf甲900本文3.9 MBAdobe PDF見る/開く
02甲900本文の要旨.pdf甲900本文の要旨115.69 kBAdobe PDF見る/開く
03甲900審査要旨.pdf甲900審査要旨201.48 kBAdobe PDF見る/開く
04甲900Supplementary Figure1.pdf甲900Supplementary Figure1255.77 kBAdobe PDF見る/開く
05甲900Supplementary Figure2.pdf甲900Supplementary Figure2260.83 kBAdobe PDF見る/開く
06甲900Supplementary Table.pdf甲900Supplementary Table124.2 kBAdobe PDF見る/開く

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