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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/4014

Title: Intermittent Hypoxia Upregulates the Renin and Cd38 mRNAs in Renin-Producing Cells via the Downregulation of miR-203.
Other Titles: 間歇的低酸素によりレニン産生細胞では microRNA-203 の発現低下を介して Renin と Cd38 の発現上昇が起こる
Authors: Takeda, Yoshinori
Itaya-Hironaka, Asako
Yamauchi, Akiyo
Makino, Mai
Sakuramoto-Tsuchida, Sumiyo
Ota, Hiroyo
Kawaguchi, Ryuji
Takasawa, Shin
Keywords: CD38
cyclic ADP-ribose
intermittent hypoxia
juxtaglomerular cell
renin-angiotensin system
sleep apnea syndrome
Issue Date: 19-Sep-2021
Publisher: MDPI
Citation: International journal of molecular sciences Vol.22 No.18 Article No.10127 (2021 Sep)
Abstract: Sleep apnea syndrome is characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), and it is a known risk factor for hypertension. The upregulation of the renin-angiotensin system has been reported in IH, and the correlation between renin and CD38 has been noted. We exposed human HEK293 and mouse As4.1 renal cells to experimental IH or normoxia for 24 h and then measured the mRNA levels using a real-time reverse transcription polymerase chain reaction. The mRNA levels of Renin (Ren) and Cd38 were significantly increased by IH, indicating that they could be involved in the CD38-cyclic ADP-ribose signaling pathway. We next investigated the promotor activities of both genes, which were not increased by IH. Yet, a target mRNA search of the microRNA (miRNA) revealed both mRNAs to have a potential target sequence for miR-203. The miR-203 level of the IH-treated cells was significantly decreased when compared with the normoxia-treated cells. The IH-induced upregulation of the genes was abolished by the introduction of the miR-203 mimic, but not the miR-203 mimic NC negative control. These results indicate that IH stress downregulates the miR-203 in renin-producing cells, thereby resulting in increased mRNA levels of Ren and Cd38, which leads to hypertension.
Description: 博士(医学)・甲第831号・令和4年3月15日
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/10564/4014
ISSN: 14220067
Academic Degrees and number: 24601A831
Degree-granting date: 2022-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2021年度

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