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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/4008

Title: Human Adipose-Derived Mesenchymal Stem Cells Ameliorate Elastase-Induced Emphysema in Mice by Mesenchymal-Epithelial Transition.
Other Titles: ヒト脂肪由来間葉型幹細胞は、間葉上皮転換によりエラスターゼ誘発マウス肺気腫を改善する
Authors: Fujioka, Nobuhiro
Kitabatake, Masahiro
Ouji-Sageshima, Noriko
Ibaraki, Takahiro
Kumamoto, Makiko
Fujita, Yukio
Hontsu, Shigeto
Yamauchi, Motoo
Yoshikawa, Masanori
Muro, Shigeo
Ito, Toshihiro
Keywords: chronic obstructive pulmonary disease
mesenchymal–epithelial transition
adipose derived mesenchymal stem cell
pulmonary function test
Issue Date: 8-Oct-2021
Publisher: DOVE Medical Press
Citation: International journal of chronic obstructive pulmonary disease Vol.16 p.2783-2793 (2021 Oct)
Abstract: Purpose: Chronic obstructive pulmonary disease (COPD) is a worldwide problem because of its high prevalence and mortality. However, there is no fundamental treatment to ameliorate their pathological change in COPD lung. Recently, adipose-derived mesenchymal stem cells (ADSCs) have attracted attention in the field of regenerative medicine to repair damaged organs. Moreover, their utility in treating respiratory diseases has been reported in some animal models. However, the detailed mechanism by which ADSCs improve chronic respiratory diseases, including COPD, remains to be elucidated. We examined whether human ADSCs (hADSCs) ameliorated elastase-induced emphysema and whether hADSCs differentiated into alveolar epithelial cells in a murine model of COPD. Methods: Female SCID-beige mice (6 weeks old) were divided into the following four groups according to whether they received an intratracheal injection of phosphate-buffered saline or porcine pancreatic elastase, and whether they received an intravenous injection of saline or hADSCs 3 days after intratracheal injection; Control group, hADSC group, Elastase group, and Elastase-hADSC group. We evaluated the lung function, assessed histological changes, and compared gene expression between hADSCs isolated from the lung of Elastase-hADSC group and naïve hADSCs 28 days after saline or elastase administration. Results: hADSCs improved the pathogenesis of COPD, including the mean linear intercept and forced expiratory volume, in an elastase-induced emphysema model in mice. Furthermore, hADSCs were observed in the lungs of elastase-treated mice at 25 days after administration. These cells expressed genes related to mesenchymal-epithelial transition and surface markers of alveolar epithelial cells, such as TTF-1, β-catenin, and E-cadherin. Conclusion: hADSCs have the potential to improve the pathogenesis of COPD by differentiating into alveolar epithelial cells by mesenchymal-epithelial transition.
Description: 博士(医学)・甲第825号・令和4年3月15日
© 2021 Fujioka et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms. php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
URI: http://hdl.handle.net/10564/4008
ISSN: 11769106
DOI: https://doi.org/10.2147/COPD.S324952
Academic Degrees and number: 24601A825
Degree-granting date: 2022-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2021年度

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