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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3761

Title: Betulinic acid prevents lesion growth after venous ischemia in rats.
Other Titles: Betulinic acidのラット静脈虚血進行抑制効果について
Authors: Horiuchi, Kaoru
Nakagawa, Ichiro
Heimann, Axel
Alessandri, Beat
Li, Huige
Kempski, Oliver
Keywords: betulinic acid
venous ischemia
introsative stress
oxidative stress
Issue Date: 30-Nov-2019
Publisher: 奈良医学会
Citation: Journal of Nara Medical Association Vol.70 No.4,5,6 p.45-56 (2019.11)
Abstract: Background/Objectives: Betulinic acid is a component of a Chinese traditional herb that upregulates endothelial nitric oxide (NO) synthase (eNOS) and reduces NADPH expression. Increased production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after cerebral arterial ischemia is a major cause of neuronal damage. Local venous ischemia can arise that slowly damages surrounding brain tissue during surgical procedures. The present study investigated the effects on infarct size when betulinic acid was administered after inducing two-vein occlusion (2VO) with a slowly developing lesion in rats. Methods: We elicited 2VO in 18 male Wistar rats by the photochemical thrombosis of two adjacent cortical veins combined with KCL-induced cortical spreading depression (CSD). The rats were then randomized into groups (n=9 each) to receive either dimethyl sulfoxide (DMSO) vehicle (control) or betulinic acid (30mg/kg/day; n=9) by daily gavage for seven days, and then infarct volume and 3-nitrotyrosine expression were assessed. Results: Daily administration of betulinic acid for seven days significantly reduced infarct volume from 3.81 ± 0.7 to 1.90 ± 0.3mm³ (p=0.017). Physiological data and regional cerebral blood flow did not significantly differ between the two groups during the study. We found 3-nitrotyrosine expression within the border zone of the infarct area and cleaved caspase-3 only within the lesion at the ipsilateral hemisphere in both groups. However, 3-nitrotyrosine/caspase-3 expression did not significantly differ at any time between the groups. Conclusions: Betulinic acid reduced neuronal damage in a rat model of cerebral venous ischemia.
Description: 博士(医学)・乙第1460号・令和2年6月30日
URI: http://hdl.handle.net/10564/3761
ISSN: 13450069
Academic Degrees and number: 24601B1460
Degree-granting date: 2020-06-30
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:Vol.70 No.4,5,6

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