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http://hdl.handle.net/10564/3742
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タイトル: | Immunohistochemical expression of CD44v9 and 8-OHdG in ovarian endometrioma and the benign endometriotic lesions adjacent to clear cell carcinoma. |
その他のタイトル: | 卵巣子宮内膜症性嚢胞の癌化におけるCD44v9と8-OHdGの発現 |
著者: | Niiro, Emiko![](/dspace/image/metalink/link_ja.gif) Kawahara, Naoki![](/dspace/image/metalink/link_ja.gif) Yamada, Yuki![](/dspace/image/metalink/link_ja.gif) Yoshimoto, Chiharu![](/dspace/image/metalink/link_ja.gif) Shimada, Keiji![](/dspace/image/metalink/link_ja.gif) Sudo, Tamotsu Kobayashi, Hiroshi![](/dspace/image/metalink/link_ja.gif) |
キーワード: | 8-OHdG CD44v9 clear cell carcinoma of the ovary endometriosis immunohistochemistry |
発行日: | 2019年11月 |
出版者: | Wiley |
引用: | The journal of obstetrics and gynaecology research Vol.45 No.11 p.2260-2266 (2019 Nov) |
抄録: | Aim: Expression of CD44 variant isoforms (CD44v) promotes the synthesis of reduced glutathione and contributes to reactive oxygen species defense through up-regulation of the intracellular antioxidant. The aim of the study was to investigate the expression of CD44v9 and oxidative DNA damage marker, 8-OHdG, in benign ovarian endometrioma (OE) and OE harboring clear cell carcinomas (CCC). Methods: A retrospective study was performed at the Department of Gynecology, Nara Medical University hospital from January 2006 to December 2012. Patients with histologically confirmed benign OE (n = 27) and OE harboring areas of CCC (n = 8) were selected. Tissue samples were immunohistochemically analyzed for the presence of CD44v9 and 8-OHdG using avidin-biotin complex method. Results: CD44v9 was located on the cell membrane of endometriotic epithelial cells and expressed in 88.9% (24/27) of benign OE tissues. Only 25.0% (2/8) of benign endometriotic lesions adjacent to CCC was found to stain weakly for CD44v9. Percentage of CD44v9 positive cells was 68.5 ± 20.2% (mean ± standard deviation) of benign OE, 16.7 ± 16.5% of CCC endometriotic tissue (P < 0.001). Compared to benign OE, CCC endometriotic tissue showed a significant increase in the proportion of 8-OHdG expression (77.3 ± 22.5% vs 94.9 ± 3.0%, P = 0.049). A significant negative correlation was observed between CD44v9 status and 8-OHdG nuclear expression (r = -0.458, P = 0.006). Conclusion: Alterations in CD44v9 and 8-OHdG may be associated with malignant transformation of benign OE. |
内容記述: | 博士(医学)・乙第1452号・令和2年3月16日 © 2019 Japan Society of Obstetrics and Gynecology. This is the peer reviewed version of the following article: [https://obgyn.onlinelibrary.wiley.com/doi/full/10.1111/jog.14093], which has been published in final form at [https://doi.org/10.1111/jog.14093]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
URI: | http://hdl.handle.net/10564/3742 |
ISSN: | 13418076 |
DOI: | https://doi.org/10.1111/jog.14093 |
学位授与番号: | 24601B1452 |
学位授与年月日: | 2020-03-16 |
学位名: | 博士(医学) |
学位授与機関: | 奈良県立医科大学 |
出現コレクション: | 2019年度
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