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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3741

Title: Involvement of Receptor for Advanced Glycation Endproducts in Hypertensive Disorders of Pregnancy.
Other Titles: 妊娠高血圧症候群におけるRAGEの関与
Authors: Akasaka, Juria
Naruse, Katsuhiko
Sado, Toshiyuki
Uchiyama, Tomoko
Makino, Mai
Yamauchi, Akiyo
Ota, Hiroyo
Sakuramoto-Tsuchida, Sumiyo
Itaya-Hironaka, Asako
Takasawa, Shin
Kobayashi, Hiroshi
Keywords: hypertensive disorders of pregnancy
Issue Date: Nov-2019
Publisher: MDPI
Citation: International journal of molecular sciences Vol.20 No.21 Article No.5462 (2019 Nov)
Abstract: Preeclampsia/hypertensive disorders of pregnancy (PE/HDP) is a serious and potentially life-threatening disease. Recently, PE/HDP has been considered to cause adipose tissue inflammation, but the detailed mechanism remains unknown. We exposed human primary cultured adipocytes with serum from PE/HDP and healthy controls for 24 h, and analyzed mRNA expression of several adipokines, cytokines, and ligands of the receptor for advanced glycation endproducts (RAGE). We found that the mRNA levels of interleukin-6 (IL-6), C-C motif chemokine ligand 2 (CCL2), high mobility group box 1 (HMGB1), and RAGE were significantly increased by the addition of PE/HDP serum. Among RAGE ligands, advanced glycation endproducts (AGE) and HMGB1 increased mRNA levels of IL-6 and CCL2 in SW872 human adipocytes and mouse 3T3-L1 cells. The introduction of small interfering RNA for RAGE (siRAGE) into SW872 cells abolished the AGE- and HMGB1-induced up-regulation of IL-6 and CCL2. In addition, lipopolysaccharide (LPS), a ligand of RAGE, increased the expression of IL-6 and CCL2 and siRAGE attenuated the LPS-induced expression of IL-6 and CCL2. These results strongly suggest that the elevated AGE, HMGB1, and LPS in pregnant women up-regulate the expression of IL-6 and CCL2 via the RAGE system, leading to systemic inflammation such as PE/HDP.
Description: 博士(医学)・乙第1451号・令和2年3月16日
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/10564/3741
ISSN: 14220067
DOI: https://doi.org/10.3390/ijms20215462
Academic Degrees and number: 24601B1451
Degree-granting date: 2020-03-16
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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