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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3720

Title: Intermittent Hypoxia Up-Regulates CCL2, RETN, and TNFα mRNAs in Adipocytes via Down-regulation of miR-452.
Other Titles: 間欺的低酸素被曝は、脂肪細胞においてmicro RNA-452の発現低下を介してCCL2、Resistin、TNFαの発現上昇を引き起こす
Authors: Uchiyama, Tomoko
Itaya-Hironaka, Asako
Yamauchi, Akiyo
Makino, Mai
Sakuramoto-Tsuchida, Sumiyo
Shobatake, Ryogo
Ota, Hiroyo
Takeda, Maiko
Ohbayashi, Chiho
Takasawa, Shin
Keywords: adipokine
intermittent hypoxia
sleep apnea syndrome
Issue Date: 22-Apr-2019
Publisher: MDPI
Citation: International journal of molecular sciences Vol.20 No.8 Article No.1960 (2019 Apr)
Abstract: Sleep apnea syndrome (SAS), characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia [IH]), is a risk factor for insulin resistance. Recently, IH is considered to independently cause adipose tissue inflammation/dysfunction, leading to worsening insulin resistance; however, the detailed mechanism remains unknown. We exposed mouse 3T3-L1 and human SW872 adipocytes to experimental IH or normoxia for 24 h, and analyzed mRNA expression of several adipokines. We found that the mRNA levels of RETN, TNFα, and CCL2 in SW872 and 3T3-L1 adipocytes were significantly increased by IH, whereas the promoter activities of these genes were not increased. A target mRNA search of microRNA (miR)s revealed that all human mRNAs have a potential target sequence for miR-452. The miR-452 level of IH-treated cells was significantly decreased compared to normoxia-treated cells. MiR-452 mimic and non-specific control RNA (miR-452 mimic NC) were introduced into SW872 cells, and the IH-induced up-regulation of the genes was abolished by introduction of the miR-452 mimic but not by the miR-452 mimic NC. These results indicate that IH stress down-regulates the miR-452 in adipocytes, resulting in increased levels of RETN, TNFα, and CCL2 mRNAs, leading to insulin resistance in SAS patients.
Description: 博士(医学)・甲第729号・令和2年3月16日
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/10564/3720
ISSN: 14220067
Academic Degrees and number: 24601A729
Degree-granting date: 2020-03-16
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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