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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3674

Title: Expression of REG family genes in human inflammatory bowel diseases and its regulation.
Other Titles: 炎症性腸疾患におけるREG遺伝子ファミリーの発現と制御
Authors: Tsuchida, Chikatsugu
Sakuramoto-Tsuchida, Sumiyo
Takeda, Maiko
Itaya-Hironaka, Asako
Yamauchi, Akiyo
Misu, Masayasu
Shobatake, Ryogo
Uchiyama, Tomoko
Makino, Mai
Pujol-Autonell, Irma
Vives-Pi, Marta
Ohbayashi, Chiho
Takasawa, Shin
Keywords: REG family genes
Crohn's disease
Ulcerative colitis
Celiac disease
Issue Date: 23-Oct-2017
Publisher: Elsevier
Citation: Biochemistry and biophysics reports Vol.12 p.198-205 (2017 Oct)
Abstract: The pathophysiology of inflammatory bowel disease (IBD) reflects a balance between mucosal injury and reparative mechanisms. Some regenerating gene (Reg) family members have been reported to be expressed in Crohn's disease (CD) and ulcerative colitis (UC) and to be involved as proliferative mucosal factors in IBD. However, expression of all REG family genes in IBD is still unclear. Here, we analyzed expression of all REG family genes (REG Iα, REG Iβ, REG III, HIP/PAP, and REG IV) in biopsy specimens of UC and CD by real-time RT-PCR. REG Iα, REG Iβ, and REG IV genes were overexpressed in CD samples. REG IV gene was also overexpressed in UC samples. We further analyzed the expression mechanisms of REG Iα, REG Iβ, and REG IV genes in human colon cells. The expression of REG Iα was significantly induced by IL-6 or IL-22, and REG Iβ was induced by IL-22. Deletion analyses revealed that three regions (- 220 to - 211, - 179 to - 156, and - 146 to - 130) in REG Iα and the region (- 274 to- 260) in REG Iβ promoter were responsible for the activation by IL-22/IL-6. The promoters contain consensus transcription factor binding sequences for MZF1, RTEF1/TEAD4, and STAT3 in REG Iα, and HLTF/FOXN2F in REG Iβ, respectively. The introduction of siRNAs for MZF1, RTEF1/TEAD4, STAT3, and HLTF/FOXN2F abolished the transcription of REG Iα and REG Iβ. The gene activation mechanisms of REG Iα/REG Iβ may play a role in colon mucosal regeneration in IBD.
Description: 博士(医学)・乙第1438号・令和元年9月27日
© 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
URI: http://hdl.handle.net/10564/3674
ISSN: 24055808
Academic Degrees and number: 24601B1438
Degree-granting date: 2019-09-27
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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