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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3593

Title: The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo.
Other Titles: 上顎の形態発生におけるWnt signaling pathwayの役割
Authors: Shimomura, Tadahiro
Kawakami, Masayoshi
Tatsumi, Kouko
Tanaka, Tatsuhide
Morita-Takemura, Shoko
Kirita, Tadaaki
Wanaka, Akio
Keywords: Wnt signaling
Dickkopf-1
N-cadherin
cleft lip
chick embryo
Issue Date: Feb-2019
Publisher: 日本組織細胞化学会(The Japan Society of Histochemistry and Cytochemistry)
Citation: Acta histochemica et cytochemica Vol.52 No.1 p.19-26 (2019 Feb)
Abstract: Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. To examine whether Wnt signaling can influences upper jaw development, we applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the treated side, and skeletal staining revealed hypoplasia of the premaxilla and palatine bone as a result of Dkk-1-soaked bead implantation. 5-bromo-2'-deoxyuridine (BrdU)-positive cell numbers in the treated maxillary prominence were significantly lower at both 24 and 48 hr after implantation. Down-regulation of the expression of Bmp4, Tbx22, Sox9, and Barx1 was confirmed in the maxillary prominence treated with Dkk-1, which indicated that the deformity of the maxillary bone was controlled by gene targets of the Wnt signaling pathway. Expression of N-cadherin was seen immunohistochemically in the maxillary prominences of embryos at 6 hr and increased at 24 hr after AL treatment. Wnt signaling enhanced by AL or Wnt3a up-regulated the expression levels of Msx1, Bmp4, Tbx22, Sox9, and Barx1. Our data suggest that the Wnt signaling pathway regulates maxillary morphogenesis and growth through Bmp4, Tbx22, Sox9, and Barx1. Wnt signaling might regulate N-cadherin expression via Msx1, resulting in cell aggregation for osteochondrogenesis.
Description: 博士(医学)・乙第1430号・平成31年6月26日
© 2019 The Japan Society of Histochemistry and Cytochemistry
Copyright: © 2019 The author. This is an open access article distributed under the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, istribution, and reproduction in any medium, provided the original work is properly cited.
J-STAGEへのリンク : http://dx.doi.org/10.1267/ahc.18038
URI: http://hdl.handle.net/10564/3593
ISSN: 00445991
Academic Degrees and number: 24601B1430
Degree-granting date: 2019-06-26
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2019年度

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