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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3560

Title: A Sensitive and Time-Saving Method for the Diagnosis of Drowning by Multiplex PCR.
Other Titles: Multiplex PCRを用いた簡便で感度の高い溺死診断法の開発
Authors: Nakanishi, Mari
Nakayama, Akifumi
Kasuda, Shogo
Kudo, Risa
Yuui, Katsuya
Ishitani, Akiko
Hatake, Katsuhiko
Keywords: Diagnosis of drowning
Multiplex PCR
Aquatic bacteria
Respiratory tract bacteria
Issue Date: 31-Dec-2018
Publisher: 奈良医学会
Citation: Journal of Nara Medical Association Vol.69 No.4,5,6 p.77-85 (2018.12)
Abstract: For diagnosing death due to drowning, the method of acid digestion of diatoms is widely used to detect plankton in the organs of the corpse. However, the method is limited by its being complex, hazardous, time-consuming, and insufficiently sensitive. We therefore, developed a novel simple method to diagnose death due to drowning, and determined the location of drowning by detecting genes of representative bacteria in the environment. To procure all the information in one step, the multiplex PCR method was designed. For the diagnosis of drowning, the genes of upper respiratory indigenous bacteria, Streptococcus salivarius and Streptococcus sanguinis were used as indicators. For detection of the location of drowning, Aeromonas hydrophila and Microcystis aeruginosa were used as indicators of freshwater, and Vibrio harveyi as an indicator of seawater. A set of primers was designed for multiplex PCR. to amplify all the bacterial genes simultaneously. Using this method, 47 cases of drowning were examined, and the causes and locations of death were diagnosed.
Description: 博士(医学)・乙第1428号・平成31年3月15日
URI: http://hdl.handle.net/10564/3560
ISSN: 13450069
Academic Degrees and number: 24601B1428
Degree-granting date: 2019-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:Vol.69 No.4,5,6

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