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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3545

Title: Aquaporin-2 plays an important role in water transportation through the bladder wall in rats.
Other Titles: ラット膀胱での水吸収におけるアクアポリン-2の役割
Authors: Morizawa, Yosuke
Torimoto, Kazumasa
Hori, Shunta
Gotoh, Daisuke
Nakai, Yasushi
Miyake, Makito
Hirayama, Akihide
Tanaka, Nobumichi
Fujimoto, Kiyohide
Keywords: aquaporin
bladder
desmopressin
intravesical fluid volume
water transportion
Issue Date: Nov-2018
Publisher: John Wiley and Sons, Inc.
Citation: Neurourology and urodynamics Vol.37 No.8 p.2434-2440 (2018 Nov)
Abstract: AIM: We investigated the role of the bladder wall in permeating water, focusing on aquaporins. METHODS: Female Sprague-Dawley rats weighing 300 g were used to investigate the role of the bladder wall in saline permeation. Changes in intravesical fluid volume and sodium concentration were measured in the desmopressin acetate hydrate-loaded and control groups 3 h after administration. Bladders were resected to measure aquaporin-1, 2, and 3 gene expression using qRT-PCR. Additionally, the change of aquaporin-2 expression was measured using Western blotting and immunohistochemistry in intravesical aquaporin-2 siRNA-treated and control groups. RESULTS: Although the intravesical fluid volume and sodium concentration significantly decreased from 0 to 3 h (1.00 ± 0.00 vs 0.83 ± 0.08 mL, 157.80 ± 1.30 vs 146.8 ± 1.92 mEq/mL, P < 0.01, respectively in the control group), administration of desmopressin did not affect the extent of volume change. Aquaporin-2 expression was significantly higher in the 3-h distended bladders than in the empty bladder. Aquaporin-2 siRNA treatment suppressed aquaporin-2 expression and the change of intravesical fluid volume from 0 to 3 h (1.00 ± 0.00 and 0.99 ± 0.02 mL), which was related to the suppression of sodium concentration change in comparison with control siRNA treatment (149.6 ± 2.4 vs 143.6 ± 3.67 mEq/mL, P < 0.05). CONCLUSIONS: The rat urinary bladder absorbs water and salts under the full-filled condition. Aquaporin-2 plays an important role in the transport of water, accompanied by sodium concentration change. We demonstrated a part of the bladder absorption mechanism, which may lead to development of a new method for regulating bladder storage function.
Description: 博士(医学)・甲第697号・平成31年3月15日
© 2018 Wiley Periodicals, Inc.
This is the pre-peer reviewed version of the following article: [https://onlinelibrary.wiley.com/doi/full/10.1002/nau.23715], which has been published in final form at [http://dx.doi.org/10.1002/nau.23715]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
発行元が定める登録猶予期間終了の後、本文を登録予定(2019.11)
URI: http://hdl.handle.net/10564/3545
ISSN: 07332467
Academic Degrees and number: 24601A697
Degree-granting date: 2019-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2018年度

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