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01 奈良県立医科大学 >
012 大学院 >
0122 学位請求論文 >
01221 博士論文(医学) >
2017年度 >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10564/3426
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Title: | Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium. |
Other Titles: | 子宮筋層の内外層に発生する子宮腺筋症おける、それぞれの組織学的特徴 |
Authors: | Kishi, Yohei Shimada, Keiji Fujii, Tomomi Uchiyama, Tomoko Yoshimoto, Chiharu Konishi, Noboru Ohbayashi, Chiho Kobayashi, Hiroshi |
Issue Date: | 18-Dec-2017 |
Publisher: | Public Library of Science |
Citation: | PloS one Vol.12 No.12 Article No.e0189522 (2017 Dec) |
Abstract: | OBJECTIVE: To estimate the phenotypic characterization of fibrotic process in adenomyosis occurring at the inner or the outer myometrium. METHODS: Eight cases of adenomyosis occurring at the inner myometrium (Subtype I) and 10 cases of adenomyosis occurring at the outer myometrium (Subtype II), and 10 normal counterparts were used in this study. A immunohistochemical study for smooth muscle cells (SMCs) was performed using cytoskeletal proteins, Type I and III collagen, TGF-β and its signaling molecules. RESULTS: An increased expression of Type I collagen was observed in the extracellular matrix of adenomyotic foci. In normal uteri, immunostaining of SMC differentiation marker proteins (Desmin, Smoothelin, Myosin heavy chain (MHC)) were absent or only found in low numbers at the inner myometrium, while all of these marker proteins were clearly stained at the outer myometrium. In both types of adenomyotic foci, Desmin, Smoothelin, and MHC commonly showed a negative staining at the adjacent area to the glands. A significant staining of Non-muscle myosin IIB, TGF-β, and phosphorylated TGF-β type I receptors were found only at the SMCs of Subtype II adenomyosis. The Smad3/2 ratio of Subtype II adenomyosis was significantly higher than that of Subtype I. CONCLUSIONS: The inner myometrium of normal uteri was composed of undifferentiated phenotypes of SMCs, while the outer myometrium was composed of terminally differentiated SMCs. Various fibrotic processes have been suggested in the development of uterine adenomyosis. Distinct expression patterns of fibrosis related proteins have been shown to be implicated with differences in the subtypes of adenomyosis. |
Description: | 博士(医学)・甲第681号・平成30年3月15日 Copyright: © 2017 Kishi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/10564/3426 |
ISSN: | 19326203 |
Academic Degrees and number: | 24601A681 |
Degree-granting date: | 2018-03-15 |
Degree name: | 博士(医学) |
Degree-granting institutions: | 奈良県立医科大学 |
Appears in Collections: | 2017年度
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