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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3426

Title: Phenotypic characterization of adenomyosis occurring at the inner and outer myometrium.
Other Titles: 子宮筋層の内外層に発生する子宮腺筋症おける、それぞれの組織学的特徴
Authors: Kishi, Yohei
Shimada, Keiji
Fujii, Tomomi
Uchiyama, Tomoko
Yoshimoto, Chiharu
Konishi, Noboru
Ohbayashi, Chiho
Kobayashi, Hiroshi
Issue Date: 18-Dec-2017
Publisher: Public Library of Science
Citation: PloS one Vol.12 No.12 Article No.e0189522 (2017 Dec)
Abstract: OBJECTIVE: To estimate the phenotypic characterization of fibrotic process in adenomyosis occurring at the inner or the outer myometrium. METHODS: Eight cases of adenomyosis occurring at the inner myometrium (Subtype I) and 10 cases of adenomyosis occurring at the outer myometrium (Subtype II), and 10 normal counterparts were used in this study. A immunohistochemical study for smooth muscle cells (SMCs) was performed using cytoskeletal proteins, Type I and III collagen, TGF-β and its signaling molecules. RESULTS: An increased expression of Type I collagen was observed in the extracellular matrix of adenomyotic foci. In normal uteri, immunostaining of SMC differentiation marker proteins (Desmin, Smoothelin, Myosin heavy chain (MHC)) were absent or only found in low numbers at the inner myometrium, while all of these marker proteins were clearly stained at the outer myometrium. In both types of adenomyotic foci, Desmin, Smoothelin, and MHC commonly showed a negative staining at the adjacent area to the glands. A significant staining of Non-muscle myosin IIB, TGF-β, and phosphorylated TGF-β type I receptors were found only at the SMCs of Subtype II adenomyosis. The Smad3/2 ratio of Subtype II adenomyosis was significantly higher than that of Subtype I. CONCLUSIONS: The inner myometrium of normal uteri was composed of undifferentiated phenotypes of SMCs, while the outer myometrium was composed of terminally differentiated SMCs. Various fibrotic processes have been suggested in the development of uterine adenomyosis. Distinct expression patterns of fibrosis related proteins have been shown to be implicated with differences in the subtypes of adenomyosis.
Description: 博士(医学)・甲第681号・平成30年3月15日
Copyright: © 2017 Kishi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/10564/3426
ISSN: 19326203
Academic Degrees and number: 24601A681
Degree-granting date: 2018-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2017年度

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