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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3422

Title: Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats.
Other Titles: PDE5阻害薬であるタダラフィルは糖尿病ラットの膀胱血流を改善し、初期段階に低下する下部尿路機能を回復する
Authors: Gotoh, Daisuke
Torimoto, Kazumasa
Tatsumi, Yoshihiro
Hori, Shunta
Yamada, Atsushi
Miyake, Makito
Morizawa, Yosuke
Aoki, Katsuya
Tanaka, Nobumichi
Hirayama, Akihide
Fujimoto, Kiyohide
Keywords: bladder
phosphodiesterase 5
Issue Date: 7-Aug-2017
Publisher: Wiley
Citation: Neurourology and urodynamics Vol.37 No.2 p.666-672 (2018 Feb)
Abstract: AIMS: To investigate the effect of tadalafil on bladder blood flow and lower urinary tract function in a rat model of diabetes. MATERIALS AND METHODS: We studied female Sprague-Dawley rats and induced diabetes in some using a single intraperitoneal injection of streptozotocin. We divided the rats into nondiabetes (ND), diabetes (D), and diabetes with tadalafil (DT) groups. The rats were raised for an additional 7 weeks after diabetes induction. The DT group received oral tadalafil (2 mg/kg/day) for 7 days before the experiments. At 7 weeks after diabetes induction, we performed cystometry, resected the bladders for immunohistochemistry (hypoxia-inducible factor-1α [HIF-1α] and 8-oxo-2'-deoxyguanosine [8-OHdG] staining), and measured bladder blood supply using a laser blood flow meter. RESULTS: The opening pressure, when the urethra opens and urine flow starts, was significantly lower in the DT group than in the D group (24.9 ± 5.9 vs 43.6 ± 12.3 cmH2 O). The inter-contraction interval was significantly longer in the D group than in the ND and DT groups (1566.2 ± 168.7 vs 702.9 ± 165.2 and 787.4 ± 148.8 s). Immunohistochemistry showed positive staining of the urothelial layer for both HIF-1α and 8-OHdG in the D group, but not in the ND or DT groups. Bladder blood flow was significantly lower in the D group than in the ND or DT groups. CONCLUSIONS: Tadalafil improves bladder blood supply and lower urinary tract function in diabetic rats. Tadalafil may be a promising drug that restores lower urinary tract dysfunction in the early phase of diabetes.
Description: 博士(医学)・甲第677号・平成30年3月15日
© 2017 Wiley Periodicals, Inc.
This is the peer reviewed version of the following article : which has been published in final form at http://dx.doi.org/10.1002/nau.23372. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
URI: http://hdl.handle.net/10564/3422
ISSN: 07332467
Academic Degrees and number: 24601A677
Degree-granting date: 2018-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2017年度

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