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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3391

Title: Calcium Concentration in Culture Medium as a Nondestructive and Rapid Marker of Osteogenesis.
Other Titles: 非破壊的で迅速に骨形成を評価できるマーカーとしての培養液中カルシウム濃度について
Authors: Tanikake, Yohei
Akahane, Manabu
Furukawa, Akira
Tohma, Yasuaki
Inagaki, Yusuke
Kira, Tsutomu
Tanaka, Yasuhito
Keywords: Nondestructive
Osteogenic marker
Calcium concentration
Mesenchymal stromal cells
Tissue engineering
Issue Date: 9-Jun-2017
Publisher: Cognizant, LLC. / SAGE Publishing
Citation: Cell transplantation Vol.26 No.6 p.1067-1076 (2017 Jun)
Abstract: Artificial bones made of β-tricalcium phosphate (β-TCP) combined with bone marrow-derived mesenchymal stromal cells (BM-MSCs) are used for effective reconstruction of bone defects caused by genetic defects, traumatic injury, or surgical resection of bone tumors. However, the selection of constructs with high osteogenic potential before implantation is challenging. The purpose of this study was to determine whether the calcium concentration in BM-MSC culture medium can be used as a nondestructive and simple osteogenic marker for selecting tissue-engineered grafts constructed using β-TCP and BM-MSCs. We prepared three cell passages of BM-MSCs derived from three 7-week-old, male Fischer 344 rats; the cells were cultured in osteoinductive medium in the presence of β-TCP for 15 days. The medium was replaced with fresh medium on day 1 in culture and subsequently changed every 48 h; it was collected for measurement of osteocalcin secretion and calcium concentration by enzyme-linked immunosorbent assay and X-ray fluorescence spectrometry, respectively. After cultivation, the constructs were implanted subcutaneously into the backs of recipient rats. Four weeks after implantation, the alkaline phosphatase (ALP) activity and osteocalcin content of the constructs were measured. A strong inverse correlation was observed between the calcium concentration in the medium and the ALP activity and osteocalcin content of the constructs, with Pearson's correlation coefficients of 0.92 and 0.90, respectively. These results indicate that tissue-engineered bone with high osteogenic ability can be selected before implantation based on low calcium content of the culture medium, resulting in successful bone formation after implantation. This nondestructive, simple method shows great promise for assessing the osteogenic ability of tissue-engineered bone.
Description: 博士(医学)・乙第1411号・平成29年11月24日
Copyright © 2017 Cognizant, LLC. The articles contained in the following journals published by Cognizant, LLC are "open access articles" subject to the terms of the Creative Commons Attribution Non-Commercial (CC BY NC) license(https://creativecommons.org/licenses/by-nc/3.0/). That license permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. The articles are therefore free to read, download, cite and share with others.
URI: http://hdl.handle.net/10564/3391
ISSN: 09636897
Academic Degrees and number: 24601B1411
Degree-granting date: 2017-11-24
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2017年度

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