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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3293

Title: Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.
Other Titles: 全血流動下でのフォンビルブランド因子依存性血栓形成における組織因子の機能特性
Authors: Matsunari, Yasunori
Sugimoto, Mitsuhiko
Doi, Masaaki
Matsui, Hideto
Kawaguchi, Masahiko
Keywords: Blood flow
Fibrin
Thrombus formation
Tissue factor
Von Willebrand factor
Issue Date: Dec-2016
Publisher: Springer Nature
Citation: International journal of hematology Vol.104 No.6 p.661-668 (2016 Dec)
Abstract: Von Willebrand factor (VWF) plays an important role in mediating platelet adhesion and aggregation under high shear rate conditions. Such platelet aggregates are strengthened by fibrin-network formation triggered by tissue factor (TF). However, little is known about the role of TF in VWF-dependent thrombus formation under blood flow conditions. We evaluated TF in thrombus formation on immobilized VWF under whole blood flow conditions in an in vitro perfusion chamber system. Surface-immobilized TF amplified intra-thrombus fibrin generation significantly under both low and high shear flow conditions, while TF in sample blood showed no appreciable effects. Furthermore, immobilized TF enhanced VWF-dependent platelet adhesion and aggregation significantly under high shear rates. Neutrophil cathepsin G and elastase increased significantly intra-thrombus fibrin deposition on immobilized VWF–TF complex, suggesting the involvement of leukocyte inflammatory responses in VWF/TF-dependent mural thrombogenesis under these flow conditions. These results reveal a functional link between VWF and TF under whole blood flow conditions, in which surface-immobilized TF and VWF mutually contribute to mural thrombus formation, which is essential for normal hemostasis. By contrast, TF circulating in blood may be involved in systemic hypercoagulability, as seen in sepsis caused by severe microbial infection, in which neutrophil inflammatory responses may be active.
Description: 博士(医学)・乙第1388号・平成28年11月24日
© The Japanese Society of Hematology 2016
The original publication is available at www.springerlink.com
The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-016-2086-z
URI: http://hdl.handle.net/10564/3293
ISSN: 09255710
Academic Degrees and number: 24601B1388
Degree-granting date: 2016-11-24
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2016年度

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