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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/3184

Title: Ethanol attenuates vasorelaxation via inhibition of inducible nitric oxide synthase in rat artery exposed to interleukin-1β.
Other Titles: インターロイキン-1β暴露下のラットの血管におけるエタノールによる誘導型一酸化窒素合成酵素抑制を介した弛緩反応の抑制
Authors: Yuui, Katsuya
Kudo, Risa
Kasuda, Shogo
Hatake, Katsuhiko
Keywords: Ethanol
rat
superior mesenteric artery
interleukin-1β
relaxation response
Issue Date: 22-Oct-2015
Publisher: SAGE Publications
Citation: Human & experimental toxicology [Epub ahead of print] pii: 0960327115611944(2015 Oct 22)
Abstract: Nitric oxide produced by inducible nitric oxide synthase (iNOS) regulates sepsis-induced hypotension. During septic shock, interleukin (IL)-1β is synthesized in endothelial cells and smooth muscle cells by endotoxin. Ethanol (EtOH) suppresses endotoxin-induced hypotension. The present study aimed to elucidate the effect of EtOH on gradual relaxation and iNOS expression induced by IL-1β in isolated rat superior mesenteric arteries (SMAs). Exposure to IL-1β–induced contraction in SMA rings, followed by a gradual relaxation of phenylephrine precontracted tone. Contraction was abolished by indomethacin (IM), cycloheximide (Chx), and endothelium denudation. In contrast, the gradual relaxation was abolished by NOS inhibitors, Chx, endothelium denudation, and inhibited by EtOH (50 and 100 mM). However, IM had no effect on relaxation. Western blot analysis demonstrated that iNOS expression was induced by IL-1β and was inhibited by EtOH and endothelium denudation. Furthermore, messenger RNA expression of iNOS, but not endothelial NOS, was inhibited by EtOH. These data suggest that IL-1β–induced contraction is mediated by thromboxane A2, whereas IL-1β–induced relaxation occurs via NO derived from iNOS. The endothelium plays an important role in vasorelaxation. Taken together, EtOH inhibits IL-1β–mediated vasorelaxation by suppressing endothelium iNOS expression. This study provides the first evidence of EtOH -induced inhibition of IL-1β–mediated vasorelaxation.
Description: 博士(医学)・甲第647号・平成28年3月15日
© The Author(s)
Copyright © 2016 by SAGE Publications
The definitive version is available at " http://dx.doi.org/10.1177/0960327115611944 "
URI: http://hdl.handle.net/10564/3184
ISSN: 09603271
Academic Degrees and number: 24601A647
Degree-granting date: 2016-03-15
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2015年度

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