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01 奈良県立医科大学 >
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0122 学位請求論文 >
01221 博士論文(医学) >
2015年度 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/3078
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タイトル: | Erythropoietin attenuates intestinal inflammation and promotes tissue regeneration. |
その他のタイトル: | エリスロポエチンによる腸管に対する抗炎症、組織再生効果 |
著者: | Nakamura, Shinji Sho, Masayuki![](/dspace/image/metalink/link_ja.gif) Koyama, Fumikazu![](/dspace/image/metalink/link_ja.gif) Ueda, Takeshi Nishigori, Naoto Inoue, Takashi Nakamoto, Takayuki Fujii, Hisao![](/dspace/image/metalink/link_ja.gif) Yoshikawa, Shusaku Inatsugi, Naoki Nakajima, Yoshiyuki![](/dspace/image/metalink/link_ja.gif) |
キーワード: | cytokine erythropoietin inflammatory bowel disease intestine tissue regeneration |
発行日: | 2015年9月 |
出版者: | Taylor & Francis / Informa Healthcare |
引用: | Scandinavian journal of gastroenterology Vol.50 No.9 p.1094-1102(2015 Sep) |
抄録: | Background. The prevalence of inflammatory bowel disease (IBD) is increasing. Since patients usually need long-term treatment and suffer from reduced quality of life, there is a need to develop new therapeutic strategy. The aim of this study was to investigate the therapeutic potential of erythropoietin (EPO) for the treatment of IBD. Methods. Murine colitis was induced by 3.0% Dextran Sulfate Sodium (DSS). Recombinant human EPO (rhEPO) was given to evaluate the anti-inflammatory and regenerative effects on intestinal inflammation. The effect of rhEPO on human colon epithelial cells was also evaluated. Immunohistochemical analysis of EPO receptor was performed in human IBD tissues. Results. While about 62% of control mice with severe colitis induced by 5-day DSS died, 85% of mice treated with rhEPO survived. Histological analysis confirmed that EPO treatment reduced the colonic inflammation. Furthermore, EPO treatment significantly downregulated the local expressions of IFN-γ, TNF-α and E-selectin in the colon, suggesting that the effect was associated with inhibiting local immune activation. In a 4-day DSS-induced colitis model, rhEPO significantly improved the recovery of body weight loss compared to controls. Furthermore, proliferating cell nuclear antigen expression was significantly upregulated in the colon tissue from mice treated with rhEPO compared to controls. In addition, rhEPO increased the growth of cultured human colon epithelial cells in a dose-dependent manner. Furthermore, EPO-receptor expression was confirmed in human IBD colon tissues. Conclusion. Three major functions of EPO, hematopoiesis, anti-inflammation and regeneration, may produce significant effects on intestinal inflammation, therefore suggesting that rhEPO might be useful for IBD. |
内容記述: | 博士(医学)・乙第1364号・平成27年7月31日 © Informa UK Limited, an Informa Group Company The definitive version is available at " http://dx.doi.org/10.3109/00365521.2015.1020861 " |
URI: | http://hdl.handle.net/10564/3078 |
ISSN: | 00365521 |
DOI: | http://dx.doi.org/10.3109/00365521.2015.1020861 |
学位授与番号: | 24601B1364 |
学位授与年月日: | 2015-07-31 |
学位名: | 博士(医学) |
学位授与機関: | 奈良県立医科大学 |
出現コレクション: | 2015年度
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