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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2964

Title: Photodynamic diagnosis of shed prostate cancer cells in voided urine treated with 5-aminolevulinic acid.
Other Titles: 尿中剥離前立腺癌細胞における5-ALA を用いた光力学的診断の有用性
Authors: Nakai, Yasushi
Anai, Satoshi
Kuwada, Masaomi
Miyake, Makito
Chihara, Yoshitomo
Tanaka, Nobumichi
Hirayama, Akihide
Yoshida, Katsunori
Hirao, Yoshihiko
Fujimoto, Kiyohide
Keywords: 5-aminolevulinic acid
Prostate cancer
Photodynamic diagnosis
Urine cytology
Issue Date: 3-Aug-2014
Publisher: BioMed Central
Citation: BMC urology Vol.14 Article No.59 (2014)
Abstract: BACKGROUND:Past attempts at detecting prostate cancer (PCa) cells in voided urine by traditional cytology have been impeded by undesirably low sensitivities but high specificities. To improve the sensitivities, we evaluate the feasibility and clinical utility of photodynamic diagnosis (PDD) of prostate cancer by using 5-aminolevulinic acid (5-ALA) to examine shed prostate cancer cells in voided urine samples. METHODS:One hundred thirty-eight patients with an abnormal digital rectal exam (DRE) and/or abnormal prostate-specific antigen (PSA) levels were recruited between April 2009 and December 2010. Voided urine specimens were collected before prostate biopsy. Urine specimens were treated with 5-ALA and imaged by fluorescence microscopy and reported as protoporphyrin IX (PPIX) positive (presence of cells demonstrating simultaneous PPIX fluorescence) or PPIX negative (lack of cells demonstrating fluorescence). RESULTS:Of the 138 patients, PCa was detected on needle biopsy in 81 patients (58.7%); of these 81 patients with PCa, 60 were PPIX-positive (sensitivity: 74.1%). Although 57 patients did not harbor PCa by conventional diagnostic procedures, 17 of these at-risk patients were found to be PPIX-positive (specificity: 70.2%). PPIX-PDD was more sensitive compared with DRE and transrectal ultrasound and more specific compared with PSA and PSA density. The incidence of PPIX-PDD positivity did not increase with increasing total PSA levels, tumor stage or Gleason score.CONCLUSIONS:To our knowledge, this is the first successful demonstration of PPIX in urine sediments treated with 5-ALA used to detect PCa in a noninvasive yet highly sensitive manner. However, further studies are warranted to determine the role of PPIX-PPD for PCa detection.
Description: 博士(医学)・甲第633号・平成27年3月16日
© 2014 Nakai et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
URI: http://hdl.handle.net/10564/2964
ISSN: 14712490
DOI: http://dx.doi.org/10.1186/1471-2490-14-59
Academic Degrees and number: 24601A633
Degree-granting date: 2015-03-16
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2014年度

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