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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2783

Title: Early Fixation of Cobalt-Chromium Based Alloy Surgical Implants to Bone Using a Tissue-engineering Approach.
Other Titles: 再生骨組み込みコバルトクロム製インプラントと骨との早期固着性についての基礎的研究
Authors: Ogawa, Munehiro
Tohma, Yasuaki
Ohgushi, Hajime
Takakura, Yoshinori
Tanaka, Yasuhito
Keywords: cobalt chromium alloy
implant-bone interface
marrow mesenchymal cell
tissue engineering
Issue Date: May-2012
Publisher: MDPI : Multidisciplinary Digital Publishing Institute
Citation: International journal of molecular sciences Vo.13 No.5 p.5528-5541
Abstract: To establish the methods of demonstrating early fixation of metal implants to bone, one side of a Cobalt-Chromium (CoCr) based alloy implant surface was seeded with rabbit marrow mesenchymal cells and the other side was left unseeded. The mesenchymal cells were further cultured in the presence of ascorbic acid, β-glycerophosphate and dexamethasone, resulting in the appearance of osteoblasts and bone matrix on the implant surface. Thus, we succeeded in generating tissue-engineered bone on one side of the CoCr implant. The CoCr implants were then implanted in rabbit bone defects. Three weeks after the implantation, evaluations of mechanical test, undecalcified histological section and electron microscope analysis were performed. Histological and electron microscope images of the tissue engineered surface exhibited abundant new bone formation. However, newly formed bone tissue was difficult to detect on the side without cell seeding. In the mechanical test, the mean values of pull-out forces were 77.15 N and 44.94 N for the tissue-engineered and non-cell-seeded surfaces, respectively. These findings indicate early bone fixation of the tissue-engineered CoCr surface just three weeks after implantation.
Description: 博士(医学)・乙第1303号・平成24年11月27日
© 2012 MDPI AG
URI: http://hdl.handle.net/10564/2783
ISSN: 14220067
Appears in Collections:2012年度

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