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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2768

Title: Low concentrations of alendronate increase the local invasive potential of osteoblastic sarcoma cell lines via connexin 43 activation.
Other Titles: 低濃度アレンドロネートはコネキシン43 活性を介して造骨系肉腫細胞株の局所浸潤能を増加させる
Authors: Yoshitani, Kazuhiro
Kido, Akira
Honoki, Kanya
Akahane, Manabu
Fujii, Hiromasa
Tanaka, Yasuhito
Keywords: Low-concentration
Alendronate
Osteoblastic sarcoma
Connexin 43
Oleamide
Issue Date: 15-Jul-2011
Publisher: Elsevier
Citation: Pathology, research and practice Vol.207 No.7 p.417-422
Abstract: Bisphosphonates (BPs) are agents used for treating disorders of excessive bone resorption. In addition, due to their cell-killing activity, BPs were potent candidates for adjuvant cancer therapy. On the other hand, low-concentrations of BPs have been reported to increase cellular viability in several types of tumor cells. Therefore, we focused on the effect of BPs on cellular aggressiveness of malignant bone tumors at low concentrations. MTS assay was performed using osteosarcoma cell lines MG63 and HOS, fibrosarcoma cell line HT1080, and prostate cancer cell line PC3. All the cell lines showed toxicity at high concentrations. On the other hand, at lower concentrations, the cellular viabilities of HOS and MG63 were rather higher than those of untreated controls. Since this tendency was most evident, HOS was used for further assays, including cellular motility, bone resorption activity, and cathepsin K activity. The low-concentration of alendronate enhanced cellular viability and motility, which correlated with the expression of connexin 43 at the mRNA and protein levels. Interestingly, oleamide, a potent connexin 43 inhibitor, had an inhibitory effect on the enhanced proliferation. Our data suggest that alendronate may enhance the proliferation of osteoblastic cell line through connexin 43 activation.
Description: 博士(医学)・乙第1296号・平成24年5月28日
Copyright © 2011 Elsevier GmbH. All rights reserved.
URI: http://hdl.handle.net/10564/2768
ISSN: 03440338
Appears in Collections:2012年度

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