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Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2750

Title: Primary cerebral and cerebellar astrocytes display differential sensitivity to extracellular sodium with significant effects on apoptosis.
Other Titles: 大脳由来と小脳由来の初代培養アストロサイトは、アポトーシスに関連する細胞外ナトリウム感受性が異なる
Authors: Takeda, Tomohiko
Makinodan, Manabu
Fukami, Shin-ichi
Toritsuka, Michihiro
Ikawa, Daisuke
Yamashita, Yasunori
Kishimoto, Toshifumi
Keywords: astrocyte
sodium ion
apoptosis
hyponatraemia
central pontine myelinolysis
schizophrenia
Issue Date: Jun-2014
Publisher: Wiley-Blackwell
Citation: Cell biochemistry and function Vol.32 No.4 p.395-400
Abstract: Central pontine myelinolysis is one of the idiopathic or iatrogenic brain dysfunction, and the most common cause is excessively rapid correction of chronic hyponatraemia. While myelin disruption is the main pathology, as the diagnostic name indicates, a previous study has reported that astrocyte death precedes the destruction of the myelin sheath after the rapid correction of chronic low Na+ levels, and interestingly, certain brain regions (cerebral cortex, hippocampus, etc.) are specifically damaged but not cerebellum. Here, using primary astrocyte cultures derived from rat cerebral cortex and cerebellum, we examined how extracellular Na+ alterations affect astrocyte death and whether the response is different between the two populations of astrocytes. Twice the amount of extracellular [Na+] and voltage-gated Na+ channel opening induced substantial apoptosis in both populations of astrocytes, while, in contrast, one half [Na+] prevented apoptosis in cerebellar astrocytes, in which the Na+–Ca2+ exchanger, NCX2, was highly expressed but not in cerebral astrocytes. Strikingly, the rapid correction of chronic one half [Na+] exposure significantly increased apoptosis in cerebellar astrocytes but not in cerebral astrocytes. These results indicate that extracellular [Na+] affects astrocyte apoptosis, and the response to alterations in [Na+] is dependent on the brain region from which the astrocyte is derived.
Description: 博士(医学)・乙第1346号・平成26年12月3日
Copyright © 2014 John Wiley & Sons, Ltd.
URI: http://hdl.handle.net/10564/2750
ISSN: 02636484
Academic Degrees and number: 24601B1346
Degree-granting date: 2014-12-03
Degree name: 博士(医学)
Degree-granting institutions: 奈良県立医科大学
Appears in Collections:2014年度

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