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Vol.40 No.3 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2244

Title: アンドロゲンレセプター・金コロイド法による電子顕微鏡学的研究
Other Titles: ELECTRON MICROSCOPIC STUDY ON COLLOIDAL GOLD LABELED ANDROGEN RECEPTOR
Authors: 中山, 正成
奥田, 喜一
別府, 謙一
橋本, 研二
山本, 浩司
Keywords: androgen receptor
colloidal gold method
electron microscopic study
金コロイド
組織化学
Issue Date: 30-Jun-1989
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.40 No.3 p.315-323
Abstract: The transformed type androgen receptor was isolated from rat testis with a dihydrotestosterone Sepharose affinity column. Its physiological characters were confirmed to retain an androgen bindirig and a DNA binding activity by biochemical and histochemical studies. The molecular weight of this androgen receptor had about 68,000 daltons with gel chromatography, and was confirmed to consist of a monomer with 65,000 daltons by SDS-PAGE analysis. In an electron microscopic study with rat testis using colloidal gold labeled androgen receptor, from spermatogonia to round spermatids and Sertoli cells, they had a binding activity against the androgen receptor in their cell nuclei, so the androgen hormones may play an important role not only in Sertoli cells but also in spermatogenic cell lines.
URI: http://hdl.handle.net/10564/2244
ISSN: 04695550
13450069
Appears in Collections:Vol.40 No.3

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