アンドロゲンレセプター・金コロイド法による電子顕微鏡学的研究

Abstract

The transformed type androgen receptor was isolated from rat testis with a dihydrotestosterone Sepharose affinity column. Its physiological characters were confirmed to retain an androgen bindirig and a DNA binding activity by biochemical and histochemical studies. The molecular weight of this androgen receptor had about 68,000 daltons with gel chromatography, and was confirmed to consist of a monomer with 65,000 daltons by SDS-PAGE analysis. In an electron microscopic study with rat testis using colloidal gold labeled androgen receptor, from spermatogonia to round spermatids and Sertoli cells, they had a binding activity against the androgen receptor in their cell nuclei, so the androgen hormones may play an important role not only in Sertoli cells but also in spermatogenic cell lines.