GINMU >
01 奈良県立医科大学 >
011 医学部 >
0112 紀要 >
01121 Journal of Nara Medical Association >
Vol.40 No.5 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/2120
|
タイトル: | コロイド金標識Nontransformed type androgen receptorの組織細胞化学的研究 |
その他のタイトル: | HISTOCYTOCHEMICAL STUDY ON NONTRANSFORMED TYPE ANDROGEN RECEPTOR LABELED WITH COLLOIDAL GOLD IN RAT TESTIS |
著者: | 中山, 正成 橋本, 研二 奥田, 喜一 山本, 浩司 |
キーワード: | nontransformed androgen receptor colloidal gold post-embedding method rat testis electron microscopic study |
発行日: | 1989年10月31日 |
出版者: | 奈良医学会 |
引用: | 奈良医学雑誌 Vol.40 No.5 p.630-635 |
抄録: | As described in a previous paper, with a dihydrotestosterone affinity Sepharose 4B column the authors isolated and purified the transformed type androgen receptor in high ionic strength from the rat testis. Now, with the same affinity column we isolated the nontransformed type androgen receptor in the presence of sodium molybdate from the rat testis. The molecular weight of the receptor estimated by gel chromatography was about 300,000 daltons or more. In a histocytochemical electron microscopic study, the colloidal gold-labeled nontransformed androgen receptor had an affinity to nuclear proteins which might be constituents of the active chromatin in a Sertoli cell nuclei. The DNase treatment indicated that actin is not the target of the binding of this type of the receptor. The nontransformed androgen receptor may localize in an active chromatin region as
well as the transformed androgen receptor and regulate their hormone responsive gene in
higher order chromatin structures. |
URI: | http://hdl.handle.net/10564/2120 |
ISSN: | 04695550 13450069 |
出現コレクション: | Vol.40 No.5
|
このリポジトリに保管されているアイテムは、他に指定されている場合を除き、著作権により保護されています。
|