コロイド金標識アンドロゲンによる犬の精巣内アンドロゲンレセプターの検索

Abstract

With a colloidal gold-labeled androgen (5α-dihydrotestosterone), the androgen receptor was specifically identified in the nucleus of Sertoli cell in the canine testis by doublely stained post-embedding method in an electron microscopic study. The binding of dihydrotestosterone to the androgen receptor was partially blocked by pre-staining with testosterone. The androgen receptor was confirmed to localize at diffused chromatin only and nucleolus, condensed chromatin, interchromatin granules, perichromatin granules and nuclear sap were scarcely stained.