有鉤囊虫症の免疫学的研究 ： 有鉤囊虫症胞液抗原の抗原分析

Abstract

In this study, the antigenic components of cyst fluid antigen of Cysticercus cellulosae (Ccf Ag.) were analyzed by three methods ; two-dimensional immunoelectrophoresis (Two-DI : first electrophoresis with agarose gel, with polyacrylamide gel or with isoelectric focussing) ; gel filtration ; and western-blotting method against rabbit anti-serum and sera of cerebral cysticercosis cellulosae patients. In the three different Two-DI methods, Ccf Ag. showed at least 6 precipitin arcs (one in β to slow α, 3 in β to α and 2 in fast γ regions). Three precipitin arcs and one arc were detected at the positions of 152 kDa and 418 kDa, respectively. Three arcs and one arc were detected within the ranges of pH 4.4 to 6.4 and of pH 4.4 to 5.4, respectively. The Ccf Ag. was subjected to molecular seiving chromatography using Sephadex G-200. Protein amounts and antigenic activities were monitored by optical absorbance at 280 nm (A＿＜280＞), and antigen-binding activity with ELISA. The second peak (F-Ⅱ) of A＿＜280＞ (152 kDa) was found to have the strongest binding to the anti-Ccf specific rabbit serum (Anti-Ccf abs). Furthermore, F-Ⅱ fraction was analyzed using isoelectric focussing and subsequent western-blotting technique. The band of pⅠ 5.40 to 5.50 was immuno-stained not only with the Anti-Ccf abs but also with the sera of cerebral cysticercosis cellulosae patients. These results indicate that antigenic substance (molecular weight : 152 kDa, pⅠ : 5.40 to 5.50) was recognized as the specific and useful antigen for serodiagnosis of cysticercosis cellulosae.