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Vol.41 No.5 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/2040

Title: 全血凝固過程中のヒト第Ⅸ因子の動態
Other Titles: STUDIES ON HUMAN FACTOR Ⅸ DURING THE WHOLE BLOOD COAGULATION PROCESS
Authors: 山本, 和邦
Keywords: Factor Ⅸ activity
Factor Ⅸ antigen
Factor Ⅸ aβ
western blot
Issue Date: 31-Oct-1990
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.41 No.5 p.534-546
Abstract: Changes of FactorⅨ activity (F. Ⅸ : C) and FactorⅨ antigen (F. Ⅸ : Ag) during the whole blood coagulation process were studied in the presence of saline, Platelin, Simplastin or Thrombin. When saline, as a control, was added to whole blood, F. Ⅸ : C was gradually increased with a peak at 40 min after clotting. When various concentrations of Platelin, Simplastin or Thrombin were added to whole blood, F. Ⅸ : C was rapidly and transiently increased with a peak at 1 hr. With every addition of saline, Platelin, Simplastin or Thrombin, the levels of F. Ⅸ : Ag were gradually decreased down to 74~84% 24 hrs after clotting. Therefore, there was discrepancy between the changes of F. Ⅸ : C and F. Ⅸ : Ag. In order to investigate the proteolytic activation of F. Ⅸ during the clotting process, F. Ⅸ was partially purified by BaCl₂ adsorption and elution with sodium citrate, and then analyzed using Western blot technique. When SDS-PAGE was performed in reduced condition, a faint band with M. W. of 50,000 (F. Ⅸ aβ) appeared at 1/2~1 min after clotting. The new band, a small portion of whole F. Ⅸ, did not change for 24 hrs. These results suggest the conclusion that the proteolytic activation of F. Ⅸ during the whole blood coagulation process starts partially at 1 min after clotting and that, with every addition of Platelin, Simplastin or Thrombin, its activation is accelerated and exaggerated, but the quantity of F. Ⅸ aβ is a small portion of whole F. Ⅸ.
URI: http://hdl.handle.net/10564/2040
ISSN: 04695550
13450069
Appears in Collections:Vol.41 No.5

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