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011 医学部 >
0112 紀要 >
01121 Journal of Nara Medical Association >
Vol.41 No.5 >
このアイテムの引用には次の識別子を使用してください:
http://hdl.handle.net/10564/2040
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タイトル: | 全血凝固過程中のヒト第Ⅸ因子の動態 |
その他のタイトル: | STUDIES ON HUMAN FACTOR Ⅸ DURING THE WHOLE BLOOD COAGULATION PROCESS |
著者: | 山本, 和邦 |
キーワード: | Factor Ⅸ activity Factor Ⅸ antigen Factor Ⅸ aβ western blot |
発行日: | 1990年10月31日 |
出版者: | 奈良医学会 |
引用: | 奈良医学雑誌 Vol.41 No.5 p.534-546 |
抄録: | Changes of FactorⅨ activity (F. Ⅸ : C) and FactorⅨ antigen (F. Ⅸ : Ag) during the whole blood coagulation process were studied in the presence of saline, Platelin, Simplastin or Thrombin. When saline, as a control, was added to whole blood, F. Ⅸ : C was gradually increased with a peak at 40 min after clotting. When various concentrations of Platelin, Simplastin or Thrombin were added to whole blood, F. Ⅸ : C was rapidly and transiently increased with
a peak at 1 hr. With every addition of saline, Platelin, Simplastin or Thrombin, the levels of F. Ⅸ : Ag were gradually decreased down to 74~84% 24 hrs after clotting. Therefore, there was discrepancy between the changes of F. Ⅸ : C and F. Ⅸ : Ag. In order to investigate the proteolytic activation of F. Ⅸ during the clotting process, F. Ⅸ was partially purified by BaCl₂ adsorption and elution with sodium citrate, and then analyzed using Western blot technique. When SDS-PAGE was performed in reduced condition, a faint band with M. W. of 50,000 (F. Ⅸ aβ) appeared at 1/2~1 min after clotting. The new band, a small portion of whole F. Ⅸ, did not change for 24 hrs. These results suggest the conclusion that the proteolytic activation of F. Ⅸ during the whole blood coagulation process starts partially at 1 min after clotting and that, with every addition of Platelin, Simplastin or Thrombin, its activation is accelerated and exaggerated, but the quantity of F. Ⅸ aβ is a small portion of whole F. Ⅸ. |
URI: | http://hdl.handle.net/10564/2040 |
ISSN: | 04695550 13450069 |
出現コレクション: | Vol.41 No.5
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