アポリポ蛋白の免疫比濁法による測定とその診断的意義について

Abstract

Serum levels of apolipoproteins were determined in 386 outpatients without obvious metabolic disorder by turbidimetric immunoassay. By studies on linearity, repeatability, reproducibility and interference, this assay was confirmed to be applicable for routine laboratory tests. Clinical reference ranges in serum levels of apolipoprotein (apo) A-Ⅰ, A-Ⅱ, B, C-Ⅱ, C-Ⅲ, and E were 92. 56-173. 79, 22. 11-40. 34, 52. 53-110. 37, 1. 39-5. 44, 4. 27-12. 94 and 2. 87-6. 38 mg/dl, respectively. Also, comparative studies on skewness and coefficient of variation in distribution of values were done with apo A-1 and high-density lipoprotein (HDL) cholesterol, and apo B and low-density lipoprotein (LDL) cholesterol. The findings suggested that levels of apolipoproteins such as A-Ⅰ and B are related to particle numbers of lipoproteins such as HDL and LDL, while cholesterol content in lipoproteins is affected by qualitative changes in particles as well. In coronary heart diseases, apo B and apo B/A-Ⅰ ratios were significantly elevated and are suggested to be sensitive indicators for these diseases.