血漿von Willebrand因子の第Ⅷ因子結合能の検討

Abstract

A new analytical method which characterized the ability of plasma vWF to bind to purified F. Ⅷ in a small amount of plasma was established. In the assay, plasma vWF was immobilized and immunopurified on a polystyrene well, using an anti-vWF monoclonal antibody. Then, purified F. Ⅷ was incubated in the well and the bound F. Ⅷ was measured with a chromogenic assay. The minimum volume of plasma for the assay was 0.5 ml with 0.01 U/ml of vWF antigen. The F. Ⅷ binding to vWF processed in about 15 min, and the ability of vWF in citrated and heparinized plasma was identical. These results indicate that the assay was useful for detecting the binding ability of vWF to F. Ⅷ in plasma samples.