ヒト正常および腫瘍細胞の三次元培養による組織再構築に関する研究

Abstract

Normal and tumor cells were cultivated in three-dimensional collagen gel culture. Collagen was extracted from rat tail tendon and then sterilized with ⁶⁰Co gamma-ray irradiation. By examining the proliferation of DD762 cells (mouse mammary tumor cell lines), as well as that of normal mouse mammary gland and salivary gland cells, the collagen gel culture method was evaluated. Five kGy irradiated collagen was found to be suitable for sterilization, gelation and also the growth of normal and tumor cells. The serum-free medium was suitable for reconstruction of a duct-like structure with cells derived from gland tissues. In this culture system normal human cells (derived from salivary, thyroid and mammary glands) and tumor cells from the same glands were cultured. Histologic sections of each culture gel closely resembled the original tissue morphologically. This in vitro system is useful for sensitivity tests for various anticancer agents and for studying cell differentiation, organogenesis and tumorigenesis.