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01121 Journal of Nara Medical Association >
Vol.62 No.3,4,5 >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10564/1876
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Title: | MOLECULAR DETECTION OF MECT1-MAML2 FUSION GENE IN MUCOEPIDERMOID CARCINOMA WITH ORDINARY AND VARIANT HISTOLOGY: A STUDY USING ARCHIVAL PARAFFIN EMBEDDED TISSUE |
Authors: | Kasai, Takahiko Takeda, Maiko Enomoto, Yasunori Takano, Masato Morita, kouhei Nonomura, Akitaka |
Keywords: | mucoepidermoid carcinoma MECT1-MAML2 fusion gene RT-PCR paraffin-embedded tissue |
Issue Date: | 31-Oct-2011 |
Publisher: | 奈良医学会 奈良県立医科大学 |
Citation: | Journal of Nara Medical Association Vol.62 No.3,4,5 p.69-79 |
Abstract: | Mucoepidermoid carcinoma (MEC) has been characterized by t (11; 19)(q21;
p13). This chromosomal translocation has been recently shown to generate a MECT1-
MAML2 fusion gene. MEC can pose diagnostic challenges when they are of high-grade,
of variant histologic appearance and occurring in an unusual site. The aim of this study
was to evaluate the frequency of the MECT1-MAML2 fusion gene among primary
salivary gland MECs and extrasalivary gland MECs, together with some histological
variants and its role as a possible diagnostic adjunct, comparing the salivary gland
tumors including Warthin's tumor(WT),pleomorphic adenoma(PA),and adenoid cystic
carcinoma(ACC). Using a reverse transcription-polymerase chain (RT-PCR)-based
approach, we assayed for the MECT1-MAML2 transcript in 39 cases for which paraffin-
embedded tumor tissue with adequate RNA was available. These included 19 MECs,10
WTs, five PAs, and five ACCs. The MECT1-MAML2 fusion gene transcript was
detected in 16 (84.2%) of 19 MECs. These positive cases included two cases of MEC with
WT-like areas,a sclerosing MEC and a clear cell MEC. Three negative cases were high-
grade MECs. Two of them were not easy to distinguish from squamous cell carcinoma.
The MECT1-MAML2 fusion gene was negative in all cases of WT,PA and ACC. The
potential usefulness of MECT1-MAML2 fusion gene expression as a molecular marker in
the diagnosis of MEC is supported. |
URI: | http://hdl.handle.net/10564/1876 |
ISSN: | 13450069 |
Appears in Collections: | Vol.62 No.3,4,5
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