試作人工硝子体ポリビニールアルコールハイドロゲルに対する猿眼の病理組織学的評価

Abstract

The purpose of the present experiment is to study by clinical and histological methods the effects of artificial vitreous body made from polyvinyl alcohol hydrogel (PVA) in rhesus monkeys. Fifteen rhesus monkeys were used in the present experiment. Zero point three ml of PVA was injected into the right eye after 0.5 ml of the vitreous body was removed. As a control, 0.3 ml of physiological saline was injected into the left eye, after 0.5 ml of the vitreous body was removed. Clinical laboratory observations were done at 3 days, 1, 2, 4, 8, 12 and 16 weeks after PVA or physiological saline were injected. All eyes were removed at experiment week 16 and were used for histopathological studies. Adequate data could not be obtained in clinical observation, because of difficulty in controlling the intraocular pressure and secondary uveitis, the measurement of which required systemic anesthesia. In histological studies, granulation in outer sclera, mild infiltration of neutrophils, plasma cells and lymphocytes in the iris, granulation on the surface of the sclera, destruction of inner nuclear layer, decrease in the number of outer nuclear cells or fibrin with lymphocytes on the surface of vitreous body were seen in 8 of 12 experimental eyes. Further observations should be done on the adequacy of PVA for vitreous substitute before clinical use.