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Vol.44 No.6 >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10564/1759

Title: Nontransformed Androgen Receptorの核内受容体に関する研究
Other Titles: STUDY OF NUCLEAR ACCEPTOR OF NONTRANSFORMED ANDROGEN RECEPTOR COMPLEXES
Authors: 中山, 正成
Keywords: nontransformed androgen receptor
acceptor
histone H3
colloidal gold
Western blot
Issue Date: 31-Dec-1993
Publisher: 奈良医学会
Citation: 奈良医学雑誌 Vol.44 No.6 p.380-386
Abstract: In the presence of molybdate, nontransformed androgen receptor was isolated from rat testis cytosol fraction applied on 5α-dihydrotestosterone affinity column (FPLC system) with the one-step method. These androgen receptor complexes consisted of five major proteins (90-kDa, 80-kDa, 68-kDa, 52-kDa and 47-kDa) and reacted to 52-kDa protein with anti-androgen receptor antibody on nitrocellulose membrane, but not to 80-kDa. Recently, constituents of nontransformed androgen receptor, except for hormone binding proteins, are thought to be heat shock proteins, so that 90-kDa and also 47-kDa may be heat shock proteins. Sixty-eight-kDa is thought to be proteins contaminated through isolation method and to be bovine serum albumin. The acceptor proteins in nucleus against nontransformed androgen receptor are histone H3 protein confirmed by western blot analysis so that in the nucleus, rat testis androgen receptor may be localized on histone H3 of nucleosome core and stand by near hormone responsive elements for ligand activation processes.
URI: http://hdl.handle.net/10564/1759
ISSN: 04695550
13450069
Appears in Collections:Vol.44 No.6

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