電顕用切片からのヒストンH1A欠如DNase感受性クロマチンについて

Abstract

In this study of transcribed active chromatin, DNase sensitive chromatin was isolated from tissue block embedded in LR White Resin using TEM observation. The DNase sensitive chromatin showed that high-mobility group protein (HMG) 17 localized in them with immunoelectron microscopic study, so that they may be included as transcribed active genes. The reasons for the mechanism of chromatin dissociation from embedded resin are not yet known. SDS-PAGE analysis of chromatin proteins from sections showed a lack of histone H1A, H2A and H2B. This result is consistent with other laboratories as to transcribed active chromatin except for histone H1A and H1B, but with respect to histone H1, it is proposed that CTF/NF-1 and H1 in inactive chromatin act to change chromatin structure for transcription (see ref. 26), so that in our Laboratory, one of them, eg. histone H1B, may play an important role in acting for transcription.